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Thromb Haemost 1967; 17(01/02): 031-041
DOI: 10.1055/s-0038-1654082
Originalarbeiten - Original Articles - Travaux Originaux
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Effects on Fibrin Clot Lysis of a Trypsin Inhibitor in Peanuts (Arachis hypogaea)[*]

K Egeblad   **
1   James F. Mitchell Foundation, Institute for Medical Research, Washington, D. C. 20015, U. S. A.
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Publication Date:
26 June 2018 (online)

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Summary

Using thrombelastography the effects of a partially purified trypsin inhibitor from peanuts (Arachis hypogaea) on fibrinolysis and blood coagulation was investigated. It delays fibrinolysis induced by plasmin and by activators of plasminogen (urokinase and SK-activator). Assayed on plasmin the peanut inhibitor (PNTI) is slightly less active than soy bean trypsin inhibitor (SBTI) when equal antitryptic strengths are compared. PNTI is a more potent inhibitor of activator induced fibrinolysis than SBTI. The antifibrinolytic effect decreased in presence of human plasma. The thrombin generation in recai cified plasma was delayed by PNTI using about 30-fold the antifibrinolytic active concentration. There was only slight effect on tissue thromboplastin and no effect on thrombin in the concentrations used.

* Supported by grant HE-05020 to Dr. Tage Astrup, Director of Research, from the U. S. Public Health Service, National Institutes of Health, National Heart Institute.


** Present address : Surgical Department H., Copenhagen County Hospital, Gentofte, Denmark.


 

  • References

  • 1 Astrup T. The hemostatic balance. Thrombos. Diathes. haemorrh. (Stuttg.) 2: 347 1958;
    PubMedSearch in Google Scholar
  • 2 Astrup T, Brahman P, Ollendorf P, Rasmussen J. Haemostasis in haemophilia in relation to the haemostatic balance in the normal organism and the effect of peanuts. Thrombos. Diathes. haemorrh. (Stuttg.) 5: 329 1960;
    PubMedSearch in Google Scholar
  • 3 Astrup T, Brahman P, Sjelin K.-E. Haemophilia and the protease inhibitor in peanuts. Nature (Lond.) 194: 980 1962;
    PubMedSearch in Google Scholar
  • 4 Astrup T, Egeblad K. Thrombelastographic patterns produced by fibrinolytic agents incorporated in fibrin. Amer. J. Physiol 209: 84 1965;
    PubMedSearch in Google Scholar
  • 5 Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem 40: 346 1952;
    CrossrefPubMedSearch in Google Scholar
  • 6 Borchers R, Ackerson G. W, Kimmett L. Trypsin inhibitor. IV. Occurence in seeds of the leguminosae and other seeds. Arch. Biochem 13: 291 1947;
    PubMedSearch in Google Scholar
  • 7 Boudreaux H. B, Frampton V. L. A peanut factor for haemostasis in haemophilia. Nature (Lond.) 755: 409 1960;
    PubMedSearch in Google Scholar
  • 8 Brakman P, Sjelin K.-E, Astrup T. Is there a hemostatic effect of peanuts in hemophiolid disorders ? Thrombos. Diathes. haemorrh. (Stuttg.) 8: 442 1962;
    PubMedSearch in Google Scholar
  • 9 van Greveld S, Mochtar I. A. Haemophilia and therapy with peanuts. Ann. paediat 197: 1 1961;
    PubMedSearch in Google Scholar
  • 10 Egeblad K. Effects of epsilon-aminocaproic acid on fibrin clot lysis. Thrombos. Diathes. haemorrh. (Stuttg.) 15: 173 1965;
    PubMedSearch in Google Scholar
  • 11 Egeblad K. Effects of soybean trypsin inhibitor on fibrin clot lysis. Thrombos. Diathes. haemorrh. (Stuttg.) 16: 542 1966;
    PubMedSearch in Google Scholar
  • 12 Lassen M. Heat denaturation of plasminogen in the fibrin plate method. Acta physiol. scand 27: 371 1952;
    PubMedSearch in Google Scholar
  • 13 Nilsson I.-M. Relation mellan fettmetabolism, blodkoagulation och fibrinolys. Närings-forskning 4: 66 1960;
    PubMedSearch in Google Scholar
  • 14 Schmutzler R. Die Wirkung von Erdnüssen (Arachis hypogaea) und Erdnußextrakten auf die Gerinnungsfaktoren. Thrombos. Diathes. haemorrh. (Stuttg.) 5: 566 1961;
    PubMedSearch in Google Scholar