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Thromb Haemost 1970; 24(01/02): 214-223
DOI: 10.1055/s-0038-1654227
DOI: 10.1055/s-0038-1654227
Originalarbeiten – Original Articles – Travaux Originaux
The Adsorption of Coagulation Factors onto Phospholipids
Its Role in the Reaction Mechanism of Blood CoagulationAuthors
Further Information
Publication History
Publication Date:
28 June 2018 (online)
Summary
It is shown that factors VIII and IX react with phospholipid in a way fully analogous to the reaction of factors V and X. From this it is concluded that a complex of factors VIII and IX adsorbed onto a phospholipid micelle may be the entity that activates factor X in the intrinsic pathway. The implications of this finding for the concepts of the reaction mechanism of blood coagulation are discussed. It is shown that instead of the original 7-step cascade of Macfarlane, a 4-step cascade consisting partly of complex enzymes is more probable.
* Present address: Hôpital Universitaire St. Pierre, Service du Centre Albert Hustin, 322 Rue Haute, Bruxelles, Belgique.
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References
- 1 Altman R, Hemker H. G. Contact activation in the extrinsic blood clotting system. Thrombos. Diathes. haemorrh. (Stuttg) 18: 525 1967;
- 2 Barton P. G, Jackson G. M, Hanahan D. J. Relationship between factor V and activated factor X in the generation of prothrombinase. Nature 214: 923 1967;
- 3 Bereblum J, Chain E. An improved method for the colorimetric determination of phosphate Biochem. J 32: 295 1938;
- 4 Biggs R, Douglas A. S, Macfarlane R. G. Initial stages of blood coagulation. J. Physiol 122: 538 1953;
- 5 Biggs R, Macfarlane R. G. Human blood coagulation and its disorders. 3rd. ed. Blackwell Scientific Publications; Oxford: 1962
- 6 Biggs R, Macfarlane R. G. The reaction between factors VIII and IX to form a factor X activator. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 17: 23 1965;
- 7 Biggs R, Sharp A. A, Margolis J, Hardisty R. M, Stewart J, Davidson W. M. Defects in the early stages of blood coagulation, a report of four cases. Brit. J. Haemat 04: 177 1958;
- 8 Breckenridge R. T, Ratnoff O. D. The role of proaccelerin in human blood coagulation; evidence that proaccelerin is converted into a prothrombin-converting principle by activated Stuart-Factor ; with notes on the anti-coagulant action of soybean-trypsin-inhibitor, protamin-sulphate, and hexa-dimethrinbromide. J. of clin. Invest 44: 302-314 1965;
- 9 Breckenridge R. T, Ratnoff O. D. The activation of proaccelerin by bovine thrombokinase with notes on the antithrombic activity of phenylmethyl-sulfonylfluoride. Blood 27: 527 1966;
- 10 Cole E. R, Koppel J. L, Olwin J. H. Phospholipid-protein interactions in the formation of prothrombin activator Thrombos. Diathes. haemorrh. (Stuttg) 14: 431 1965;
- 11 Davie E. W, Ratnoff O. D. Waterfall sequence for intrinsic blood clotting. Science 145: 1310 1964;
- 12 Degeller K. The human prothrombin-activating enzyme. Thesis: Amsterdam; 1968
- 13 Esnouf M. P, Jobin F. Lipids in prothrombin conversion. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 17: 103 1965;
- 14 Esnouf M. P, Macfarlane R. G. Enzymology in the blood clotting mechanism. Advanc. Enzymol 30: 255 1968;
- 15 Haanen C, Morselt G, Schoenmakers J. Contact activation of Hageman factor, the interaction of Hageman factor and plasma thromboplastin antecedent. Thrombos. Diathes. haemorrh. (Stuttg) 17: 307 1967;
- 16 Hanahan D. J. D. Papahadjopoulos: Interaction of phospholipids with coagulation factors. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 17: 71 1965;
- 17 Hemker H. C, Esnouf P, Hemker P. W, Swart A. C. W, Marfarlane R. G. Formation of prothrombin converting activity. Nature (Lond) 215: 248 1967a
- 18 Hemker H. G, Kahn M. J. P. Reaction sequence of blood coagulation. Nature (Lond) 215: 1201 1967b
- 19 Hemker H. G, Muller A. D. Kinetic aspects of the interaction of blood clotting enzymes. V : The reaction mechanism of the extrinsic clotting system as revealed by the kinetics of onestage estimations of coagulation enzymes. Thrombos. Diathes. haemorrh. (Stuttg) 19: 368 1968;
- 20 Hemker H. G, Wimmers J. M. G. On the reaction mechanism of the extrinsic blood clotting system. Proc. 2nd meeting F. E. B. S. Vienna. 1964 A 120.
- 21 Hemker P. W, Hemker H. G. Kinetics of the interaction of blood clotting factors in a purified system; a mathematical model. Proc. Europ. Fed. Biochem. Soc. Oslo A 50: 13 1967b
- 22 Hougie C, Biggs R. o. semary, Denson K. W. E. A study of the reaction product of Factor VIII and factor IX by gel filtration. Thrombos. Diathes. haemorrh. (Stuttg) 18: 211 1967;
- 23 Jobin F, Esnouf M. P. Studies on the formation of the prothrombin converting complex. Biochem. J 102: 666 1967;
- 24 Kahn M. J. P. Studies on blood coagulation factor V. Ph. D. Thesis, University of Leiden; 1970
- 25 Kingdon H. S, Davie E. W. Further studies on the activation of factor IX by activated factor XI. Thrombos. Diathes. haemorrh. (Stuttg.) Suppl 17: 15 1965;
- 26 Klein B. Automated atomic adsorption spectrophotometry. IV: Simultaneous determination of calcium and phosphate. Clin. Chemistry 13: 1079 1967;
- 27 Macfarlane R. G. An enzyme cascade in the blood clotting mechanism and its function as a biochemical amplifier. Nature (Lond) 202: 498 1964;
- 28 Macfarlane R. G, Biggs R, Ash B. J, Denson K. W. E. The interaction of factors VIII and IX. Brit. J. Haemat 10: 530 1964;
- 29 Markward F. Versuche zur pharmakologischen Charakterisierung des Hirudins. Naunyn-Schmiedeberg’s Arch. exp. path. Pharmak 234: 516 1958;
- 30 Newcomb T. F, Hoshida M. Factor V and thrombin. Scand. J. clin. Lab. Invest. Suppl. 17 84: 6l 1965;
- 31 Nossel H. L. The activation and consumption of factor IX. Thrombos. Diathes. haemorrh. (Stuttg) 12: 505 1964;
- 32 Le Page G. A. Analysis of phosphor y lat ed intermediates. In: Umbreit W. W, Burris R. H, Stauffer J. F. (eds.), Manometric Techniques; 3rd. ed. 273. Burgess, Mineapolis: 1957
- 33 Papahadjopoulos D, Hanahan D. J. Observations on the interaction of phospholipids and certain clotting factors in prothrombin activator formation. Biochim. biophys. Acta (Amst) 90: 436 1964;
- 34 Prentice G. R, Ratnoff O. D. The action of Russell’s viper venom on factor V and the prothrombin-converting principle. Brit. J. Haemat 16: 291-302 1969;
- 35 Rapaport S. I, Schiffman S, Patch M. J, Ames S. B. The importance of activation of antihemophilic globulin and proaccelerin by traces of thrombin in the generation of intrinsic prothrombinase activity. Blood 21: 221 1963;
- 36 Rapaport S. I, Hjort P. F, Patch M. J. Further evidence that thrombin-activation of factor VIII is an essential step in intrinsic clotting. Scand. J. clin. Lab. Invest 17 (Suppl. 84) 88 1965;
- 37 Ratnoff O. D. Personal communication. 1965
- 38 Ratnoff O. D, Davie E. W. The activation of Christmas factor (factor IX) by activated plasma thromboplastin antecedent (activated factor XI). Biochemistry 01: 967 1967;
- 39 Schiffman S, Rapaport S. I, Chang M. Y. The mandatory role of lipid in the interaction of factors VIII and IX. Proc. Soc. exp. Biol. (N. Y) 123: 736 1966;
- 40 Schiffman S, Rapaport S. I, Patch M. The identification and synthesis of activated plasma thromboplastin component (PTC’). Blood 22: 733 1963;
- 41 Sen N. N, Sen R, Denson K. W. E, Biggs R. A modified method for the assay of factor IX. Thrombos. Diathes. haemorrh. (Stuttg) 18: 241 1967;
- 42 Soulier J. P, Prou-Wartelle O. New data on Hageman factor and plasma thromboplastin antecedent ; the role of contact in the initial phase of blood coagulation. Brit. J. Haemat 06: 88 1960;
- 43 Veltkamp J. J, Muller DAnnemarie, Loeliger E. A. Laboratory Investigation. In: Hemker H. C, Loeliger E. A, Veltkamp J. J. Human Blood Coagulation. 152 Leiden University Press; 1970
- 44 Waaler B. A. Contact activation in the intrinsic blood clotting system; studies on a plasma product formed on contact with glass and similar surfaces. Scand. J. clin. Lab. Invest 12 Suppl. 37 1959;