Rapid Detection of Fibrinopeptides by Bidimensional Paper Electrophoresis

Summary

A simplified method for the rapid identification of the fibrinopeptides in small amounts of supernatants of fibrin clots is described.

Using this method, we confirmed the earlier observation that Reptilase-R, a thrombin-like enzyme from the venom of Bothrops atrox, exclusively liberates fibrinopeptides A and AP. It was further demonstrated that fibrinopeptide B can be rapidly isolated from small amounts of fibrinogen or even of plasma by thrombin action on washed Reptilase clots.

• References

• 1 Blombäck B. Fibrinogen to fibrin transformation. In: Seegers WH. (Ed.) Blood clotting Enzymology. 143 Academic Press; New York: 1967
• 2 Blombäck B. The N-terminal Disulphide Knot of Human Fibrinogen. Brit. J. Haemat 17: 145 1969;
• 3 Blombäck M, Blombäck B. Purification of human fibrinogen. Arkiv Kemi 10: 415-443 1956;
• 4 Blombäck B, Blombäck M, Nilsson IM. Coagulation studies on “Reptilase”, an extract of the venom from Bothrops jararaca. Thrombos. Diathes. haemorrh. (Stuttg.). 01. 1957
• 5 Blombäck B, Blombäck M, Edman P, Hessel B. Human fibrinopeptides, isolation, characterization and structure. Biochem. biophys. Acta (Amst.) 115: 371-396 1966;
• 6 Blombäck B, Blombäck M, Hessel B, Iwanaga S. Structure of N-terminal fragments of fibrinogen and specifity of thrombin. Nature (Lond.) 215: 1445-1448 1967;
• 7 Blombäck M, Blombäck B, Mammen EF, Prasad AS. Fibrinogen Detroit - a molecular defect in the N-terminal disulfide knot of human fibrinogen?. Nature (Lond.) 218: 134-137 1968;
• 8 Copley AL, Luchini BW. The binding of human fibrinogen to native and fraction fibrins and the inhibition of polymerization of a new human fibrin monomer by fibrinogen. Life Sci 03: 1293-1305 1964;
• 9 McFarlane AS. In vivo behaviour of I¹³¹-fibrinogen. J. clin. Invest 42: 346-361 1963;
• 10 Ménaché D. La transformation du fibrinogène en fibrine. I. Action de la thrombine sur le fibrinogène. Nouv. Rev. franç. Hémat 05: 309-315 1965;
• 11 Teger Nilsson A. G, Blombäck B. Degradation of human fibrinopeptides in serum. Acta chem. scand 21: 307-309 1967;