Activation of Bovine Plasminogen by the Plasminogen Activator in Animal Tissue

Takeshi Abe; Tage Astrup

doi:10.1055/s-0038-1654497

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1960; 4(02): 201-210
DOI: 10.1055/s-0038-1654497

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Activation of Bovine Plasminogen by the Plasminogen Activator in Animal Tissue

Takeshi Abe^*

¹Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark

,

Tage Astrup

¹Biological Institute of the Carlsberg Foundation, Copenhagen, Denmark

› Author Affiliations

Abstract

Summary

1. The activation of bovine plasminogen by means of the plasminogen activator from pig heart tissue has been studied on heated fibrin and on casein.

2. Small, but varying, amounts of activator can be extracted from crude activator powder during the experiment and interfere therefore with estimations on normal, plasminogen containing fibrin.

3. The activation process is a slow process. Results obtained on heated fibrin or casein suggest that the activation is caused by a stoicheiometric reaction.

Full Text

References

References
(1) Alkjærsig N, Fletcher A. P, Sherry S. The activation of human plasminogen. II. A kinetic study of activation with trypsin, urokinase, and streptokinase. J. biol. Chem. 233: 86 1958;
(2) Astrup T. The activation of a proteolytic enzyme in blood by animal tissue. Biochem. J. 50: 5 1951;
(3) Astrup T. Fibrinokinase. Acta physiol, scand. 24: 267 1951;
(4) Astrup T. Fibrinolysis in the organism. Blood 11: 781 1956;
(5) Astrup T, Albrechtsen O. K. Estimation of the plasminogen activator and the trypsin inhibitor in animal and human tissues. Scand. J. Clin. Lab. Invest. 9: 233 1957;
(6) Astrup T, Alkjærsig N. Estimation of proteolytic enzymes by means of their fibrinolytic activity. Arch. Biochem. Biophys. 37: 99 1952;
(7) Astrup T, Müllertz S. The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346 1952;
(8) Astrup T, Stage A. Isolation of a soluble fibrinolytic activator from animal tissue. Nature 170: 929 1952;
(9) Astrup T, Sterndorff I. Fibrinolytic activity of tissue extracts and of trypsin. Nature 170: 981 1952;
(10) Astrup T, Sterndorff I. A fibrinolytic system in human milk. Proc. Soc. exp. Biol. (N. Y.) 84: 605 1953;
(11) Astrup T, Sterndorff I. The plasminogen activator in animal tissue. Acta physiol, scand 36: 250 1956;
(12) Kjeldgaard N. O, Ploug J. Urokinase, an activator of plasminogen from human urine. II. Mechanism of plasminogen activation. Biochem. Biophys. Acta 24: 283 1957;
(13) Lassen M. Heat dénaturation of plasminogen in the fibrin plate method. Acta physiol, scand. 27: 371 1952;
(14) Müllertz S. The estimation of plasmin and trypsin by means of the plate method. Acta physiol, scand. 25: 93 1952;
(15) Müllertz S. Formation and properties of the activator of plasminogen and of human and bovine plasmin. Biochem. J. 61: 424 1955;
(16) Permin P. M. The fibrinolytic activator in animal tissue. Acta physiol, scand. 21: 159 1950;