The Separation of AHF from Fibrinogen. II^[*]

Summary

A number of experiments, aimed at a greater yield of human AHF in the chromatographic separation of fibrinogen and AHF, are reported. The experiments were conducted with both anion- and cation exchangers on a basis of cellulose, and at different pH’s. Good results were obtained with ECTEOLA-cellulose, although for therapeutical purposes a somewhat greater yield would be preferable and for the determination of the properties and constitution of AHF the preparations are not pure enough. Further work is in progress.

^* This work was aided by grants from the Organization for Pure Scientific Research in the Netherlands and from the N. V. Bataafsche Petroleum My.

^** With technical assistance of Miss S. N. van Lare.

• References

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