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Thromb Haemost 1960; 4(03): 336-341
DOI: 10.1055/s-0038-1654515
Originalarbeiten — Original Articles — Travaux Originaux
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Biophysical Studies on Platelet Cofactor I Preparations[*]

Sidney Shulman   **
1   Department of Bacteriology and Immunology, University of Buffalo School of Medicine, Buffalo, New York
2   Department of Physiology and Pharmacology, Wayne State University College of Medicine, Detroit, Michigan.
,
Ricardo H. Landaburu
1   Department of Bacteriology and Immunology, University of Buffalo School of Medicine, Buffalo, New York
2   Department of Physiology and Pharmacology, Wayne State University College of Medicine, Detroit, Michigan.
,
Walter H. Seegers
1   Department of Bacteriology and Immunology, University of Buffalo School of Medicine, Buffalo, New York
2   Department of Physiology and Pharmacology, Wayne State University College of Medicine, Detroit, Michigan.
› Author AffiliationsThis investigation was supported in part by research grants H-1792 and H-2026 from the National Heart Institute, National Institutes of Health, U.S. Public Health Service.
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Publication Date:
17 June 2018 (online)

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Summary

Preparations of bovine platelet cofactor I have been examined according to biophysical methods. In the ultracentrifuge the patterns reveal a major component comprising 86% of the total and two faster moving minor components. The sedimentation constants are 6.65, 10.0, and 21.2 Svedberg units. The diffusion constant is 3.30 × 10−7 cm2 sec−1. Associating this value with the main constituent, the molecular weight is found to be 196,000. A molecular axial ratio of 12 is also obtained from these data. An axial ratio of 8 is indicated from viscosity data. The extinction coefficient at 2800 Å is 14.8.

* Manuscript received 18. 1. 1960.


** Studies carried out during the tenure of a Lederle Medical Faculty Award (1954—57). Present (additional) address: Department of Biophysics, University of Buffalo School of Medicine, Buffalo, New York.


 

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