Preparation, Stabilization and Some Properties of Purified Human Plasmin*

Daniel L Kline; Jacob B Fishman; With the technical assistance of B. A. Lage

doi:10.1055/s-0038-1654807

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1964; 11(01): 075-084
DOI: 10.1055/s-0038-1654807

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

Preparation, Stabilization and Some Properties of Purified Human Plasmin*

Daniel L Kline

¹Department of Physiology, Yale University School of Medicine, New Haven, Connecticut

,

Jacob B Fishman

¹Department of Physiology, Yale University School of Medicine, New Haven, Connecticut

,

With the technical assistance of B. A. Lage

› Author Affiliations

Abstract

Summary

1. Lysine increased the solubility, decreased the SK requirement and increased the stability of plasmin prepared from purified plasminogen by SK activation.

2. A procedure is presented for the rapid and quantitative conversion of plasminogen to plasmin and storage of the plasmin in stable form at neutral pH as a lyophilized powder.

3. Approximately 10% for the plasminogen molecule was split off during its activation. No carbohydrate was lost.

4. The plasmin isolated was homogeneous in the ultracentrifuge at pH 2.5 and was quantitatively convertible to plasminogen activator by the addition of SK.

Full Text

References

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