The Formation and “Decay” of Xlla

R. J Speer; Helen Ridgway

doi:10.1055/s-0038-1655034

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1967; 18(01/02): 259-267
DOI: 10.1055/s-0038-1655034

Originalarbeiten — Original Articles — Travaux Originaux

Schattauer GmbH

The Formation and “Decay” of Xlla^[*]

Authors

R. J Speer

¹J. K. and Susie L. Wadley Research Institute and Blood Bank and Baylor University Graduate Research Institute, Dallas, Texas (U. S. A.)
Helen Ridgway

¹J. K. and Susie L. Wadley Research Institute and Blood Bank and Baylor University Graduate Research Institute, Dallas, Texas (U. S. A.)

Abstract

Summary

Various materials and conditions have been investigated for their effect on Hageman factor activation. Fatty acid soaps, purified phospholipids, ellagic acid, platelets, and freezing and thawing were among those studied.

Under certain circumstances XIIa activity disappeared spontaneously; the factors affecting the kinetics of this “decay” have been evaluated. Successful regeneration of this “decayed” XII a has been achieved. On the basis of these observations, it is postulated that during its “decay”, XIIa reverts to XII by spontaneous molecular refolding.

Full Text

References

References
1 Campbell J, Martucci A. D, Green G. R. Plasma albumin as an acceptor of free fatty acids. Biochem. J. 93: 183 1964;
2 Connor W. E. The acceleration of thrombus formation by certain fatty acids. J. clin. Invest. 41: 1199 1962;
3 Didisheim P, Mibashan R. S. Activation of Hageman Factor (factor XII) by long-chain saturated fatty acids. Thrombos. Diathes. haemorrh. (Stuttg) 09: 346 1963;
4 Haanen C, Morselt G, Schoenmakers J. In press.
5 Margolis J. The effect of colloidal silica on blood coagulation. Aust. J. exp. Biol. 39: 249 1961;
6 Nossel H. L. The Contact Phase of Blood Coagulation. 1st. ed. 1 F. A. Davis Co; Philadelphia: 1964
7 O’Brien J. R. The effect of some fatty acids and phospholipids on blood coagulation. Brit. J. exp. Path. 38: 529 1957;
8 Pilkington T. R. E. The effect of fatty acids and detergents on the calcium clotting time of human plasma. Clin. Sci. 16: 269 1957;
9 Poole J. C. F. The effect of certain fatty acids on the coagulation of plasma in vitro. Brit. J. exp. Path. 36: 248 1955;
10 Ratnoff O. D. Activation of Hageman Factor by solutions of ellagic acid. J. Lab. clin. Med. 63: 359 1964;
11 Shafrir E, de Vries A. Studies on the clot-promoting activity of glass. J. clin. Invest. 35: 1183 1956;
12 Speer R. J, Ridgway H, Hill J. M. Activated human Hageman Factor (XII). Thrombos. Diathes. haemorrh. (Stuttg) 14: 1 1965;
13 Speer R. J, Ridgway H, Hill J. M. Lipids of the human spleen. Amer. J. Physiol. 38: 297 1962;

The Formation and “Decay” of Xlla[*]

Authors

The Formation and “Decay” of Xlla^[*]