Download PDF
Thromb Haemost 1962; 08(02): 270-275
DOI: 10.1055/s-0038-1655426
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

Molecular Weights of Factor VIII (AHF) and Factor IX (PTC) by Electron Irradiation

David L Aronson
1   Department of Health, Education and Welfare, Public Health Service, National Institutes of Health Division of Biologics Standards, Bethesda 14, Maryland
,
John W Preiss   *
1   Department of Health, Education and Welfare, Public Health Service, National Institutes of Health Division of Biologics Standards, Bethesda 14, Maryland
,
Michael W Mosesson
1   Department of Health, Education and Welfare, Public Health Service, National Institutes of Health Division of Biologics Standards, Bethesda 14, Maryland
› Author Affiliations
Further Information

Publication History

Publication Date:
14 May 2019 (online)

  PDF Download  Permissions and Reprints

Summary

The molecular weights of AHF (factor VIII) and of PTC (factor IX) have been estimated by their sensitivity to inactivation by 7 kilovolt electrons. The molecular weight of AHF was found to be 180 000 by this method and that of PTC was found to be 110 000.

* National Institute of Arthritis and Metabolic Diseases.


** Pentex Inc., Kankakee, Ill.


 

  • References

  • 1 Lea D. E. a) Actions of Radiations on Living Cells-Chapter III.. Cambridge University Press; Cambridge: 1947. b) ibid, page 25
    Google Scholar
  • 2 Biggs R, Mac farlane R. G. Human Blood Coagulation and its Disorders-Second Edition.. Thomas; Springfield, Ill.: 1957
    Google Scholar
  • 3 Cohn E. J, Strong L. E, Hughes Jr. W. L, Mulford D. J, Ash-worth J. N, Melin M, Taylor H. L. Preparations and properties of Serum and Plasma Proteins IV. A system for the separation into fractions of the protein and lipoprotein components of Biological tissues and fluids.. J. Amer. chem. Soc. 1946; 68: 459
    CrossrefPubMedGoogle Scholar
  • 4 Surgenor D. M, Wilson N. A, Henry A. S. Factor V from Human Plasma.. Thrombos. Diathes. haemorrh. 1960; 5: 1
    PubMedGoogle Scholar
  • 5 Hutchinson F. Use of Charged Particles to measure Skin Thickness and other Surface Properties.. Ann. N. Y. Acad. Sci. 1955; 59: 494
    CrossrefPubMedGoogle Scholar
  • 6 Preiss J. W. The Localization of Invertase in the Yeast Cell with Low Voltage Electrons.. Arch. Biochem. 1958; 75: 186
    CrossrefPubMedGoogle Scholar
  • 7 Lewis M. L, Ware A. G. A Simple Procedure for Separation of Prothrombin and Accelerator Globulin from Citrated Human Plasma.. Proc. Soc. exp. Biol. (N. Y.) 84: 636-640. 1953;
    CrossrefPubMedGoogle Scholar
  • 8 Hutchinson F. Inactivation of Monomolecular Layers of Bovine Serum Albumin with Deuterons.. Arch. Biochem. 1952; 41: 317
    CrossrefPubMedGoogle Scholar
  • 9 Setlow R. B, Doyle B. The Effect of Temperature on the Direct Action of Ionizing Radiation on Catalase.. Arch. Biochem. 1953; 46: 46
    CrossrefPubMedGoogle Scholar
  • 10 Braams R, Hutchinson F, Ray D. Changes in the Sensitivity of Enzymes in the Dry State to Radiation.. Nature 1958; 182: 1506
    PubMedGoogle Scholar
  • 11 Aronson D. L, Preiss J. W. Molecular Weights of Human Prothrombin and Thrombin by Electron Irradiation.. To be published.
    PubMedGoogle Scholar
  • 12 Hörder M. H, Sokal G. Der Einfluß von Faktor V auf die Thromboplastinbildung.. Acta haematologica 14: 294-302. 1955;
    CrossrefPubMedGoogle Scholar
  • 13 Hougie C. The Role of Factor V in the Formation of Blood Thromboplastin.. J. Lab. clin. Med. 1957; 50: 61-68.
    PubMedGoogle Scholar
  • 14 Hougie C, Barrow E. M, Graham J. B. Stuart Clotting Defect I. Segregation of an Hereditary Hemorrhagic State from the Heterogeneous Group Heretofore called “Stable Factor” (SPCA, Proconvertin, Factor VII) Deficiency.. J. clin. Invest. 1957; 36: 485
    CrossrefPubMedGoogle Scholar
  • 15 Wagner R. H, Theilin G. M. Antihemophilic Factor: Current Status of Purification, Chemical and Physical Characterization — Chapter I — Hemophilia and Hemo-philioid Diseases.. Brinkhous Kenneth M. University of North Carolina Press; Chapel Hill, N. C.: 1957
    Google Scholar
  • 16 Theilin G. M, Wagner R. H. Sedimentation of Plasma Antihemophilic Factor.. Arch. Biochem. 1961; 95: 70
    CrossrefPubMedGoogle Scholar
  • 17 Shulman S, Landaburu R. H, Seegers W. H. Biophysical Studies on Platelet Cofactor I.. Thrombos. Diathes. haemorrh. 1960; 4: 336
    PubMedGoogle Scholar