The Effect of Fibrinogen Concentration on Susceptibility of Clots to in vitro Clot Lysis with Plasmin

Summary

The effect of fibrinogen(fibrin) concentration on the degree of clot lysis has been is shown. These experiments demonstrate: (1) A direct correlation between degree of clot lysis and liberation of radioactive breakdown products, (2) that increasing concentrations of fibrinogen result in decreasing lysis of the clot by a standard fibrinolysin solution, (3) that lysis occurs more rapidly at pH 7.4 than at pH 7.6 and 7.8, (4) that increase in surface area results in increased lysis and (5) that increasing concentration of plasmin results in increased lysis of the clot.

^* Research Fellow, Sloan Kettering Institute.

^** Associate Professor of Clinical Surgery, Cornell Univ. and Associate, Sloan Kettering, Institute.

• References

• 1 Cliffton E. E, and Cannamela D. Fibrinolytic and proteolytic activity of a human plasminogen, prepared from Fraction III of human plasms. J. Appi. Physiol. 6: 42-50 1953;
• 2 Cliffton E. E, Cannamela D, and Grossi C. E. In vivo studies of human plasmin: Intravenous injection in dogs and rabbits. J. Appi. Physiol. 6: 143-150 1953;
• 3 Cliffton E. E, Grossi C, and Cannamela D. Lysis of thrombi produced by sodium morrhuate in the femoral vein of dogs by human plasmin. Ann. Surg. 139: 52-62 1954;
• 4 Grossi C. E, and Cliffton E. E. The lysis of arterial thrombi in rabbits and dogs by use of activated human plasminogen. Surg. 37: 794-802 1955;
• 5 Wessler S. Studies in intravascular coagulation. Ill Pathogenesis of serum induced venous thromboses. J. clin. Invest. 34: 647-651 1955;
• 6 Loomis E. Fibrinolysin and antifibrinolysin: A proteolytic enzyme system in blood-clotting and allied problems. Transactions of 5th Conference Josiah Macy Jr. Foundation 1952:280-338
• 7 Losner S, and Volk B. Fibrinogen concentrations in various clinical conditions. Am. J. Med. Sci. 232: 276-283 1956;