Lectin Stimulation of Tissue Thromboplastin Activity in Human Monocytes in vitro

Summary

Lectins (phytohaemagglutinin, concanavalin A and wheat germ agglutinin) trigger an increase in tissue thromboplastin activity of human monocytes in vitro. The presence of serum was not necessary and did not enhance the activity. The increase was inhibited by cycloheximide and actinomycin D, suggesting that de novo protein synthesis is involved.

• 1 Bjørklid E, Storm E. 1977; Purification and some properties of the protein component of tissue thromboplastin from human brain. Biochemical Journal 165: 89
• 2 Bjørklid E, Storm E, Prydz H. 1973; The protein component of human brain thromboplastin. Biochemical and Biophysical Research Communications 55: 969
• 3 Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of DNA. Biochemical Journal 62: 315
• 4 Bøyum A. 1968; Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation 21 Suppl (Suppl. 97) 77
• 5 Hvatum M, Prydz H. 1966; Studies on tissue thromboplastin. I Solubilization with sodium deoxycholate. Biochimica et biophysica acta 130: 92
• 6 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193: 265
• 7 Pitlick FA. 1975; Concanavalin A inhibits tissue factor coagulant activity. Journal of Clinical Investigation 55: 175
• 8 Prydz H, Allison AC. 1978; Tissue thromboplastin activity of isolated human monocytes. Thrombosis and Haemostasis 39: 582
• 9 Prydz H, Allison AC, Schorlemmer H-U. 1977; Further link between complement activation and blood coagulation. Nature 270: 173
• 10 Prydz H, Lyberg T, Deteix P, Allison AC. 1979; In vitro stimulation of tissue thromboplastin (Factor III) synthesis in human monocytes by immune complexes and lectins. Thrombosis Research 15: 465
• 11 Rickles FR, Levin J, Hardin JA, Barr CF, Conrad ME. 1977; Tissue factor generation by human mononuclear cells: Effects of endotoxin and dissociation of tissue factor generation from mitogenic response. Journal of Laboratory and Clinical Medicine 89: 792
• 12 Rivers RP A, Hathaway WE, Weston WL. 1975; The endotoxin-induced coagulant activity of human monocytes. British Journal of Haematology 30: 311
• 13 Rothberger H, Zimmerman TS, Spiegelberg HL, Vaughan JH. 1977; Leukocyte Procoagulant Activity. Enhancement of production in vitro by IgG and antigen-antibody complexes The Journal of Clinical Investigation 59: 549
• 14 Zacharski LR, Rosenstein R, Phillips PG. 1974; Concanavalin A inhibition of tissue factor (thromboplastin) activity. Blood 44: 783
• 15 Yam LT, Li CY, Crosby WA. 1971; Cytochemical identification of monocytes and granulocytes. American Journal of Clinical Pathology 55: 283