Mouse Trophoblast Cells Are Constitutive Producers of Thromboplastin (Factor III) In Vitro

K Dalaker; E Haug; H Prydz

doi:10.1055/s-0038-1657867

Thrombosis and Haemostasis, Inhaltsverzeichnis

Thromb Haemost 1985; 54(02): 438-441
DOI: 10.1055/s-0038-1657867

Original Article

Schattauer GmbH Stuttgart

Mouse Trophoblast Cells Are Constitutive Producers of Thromboplastin (Factor III) In Vitro

Authors

K Dalaker

¹The Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Oslo, Norway

³The Department of Obstetrics and Gynecology, Rikshospitalet, Oslo, Norway

⁴The Animal Department, Rikshospitalet, Oslo, Norway
E Haug

²The Hormone Laboratory, Aker Hospital, Oslo, Norway
H Prydz

¹The Research Institute for Internal Medicine, University of Oslo, Rikshospitalet, Oslo, Norway

Abstract

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Summary

Trophoblasts from murine placenta synthesize thromboplastin in the absence of inducing agents and a functional complement system, nor is the rate or level of synthesis enhanced by inducers. A serum factor which is destroyed/removed by addition of oxalate and subsequent dialysis appears to enhance the ability of trophoblasts to synthesize thromboplastin.

Keywords

Thromboplastin - Tissue factor - Trophoblasts - Placenta - Synthesis - Cell culture

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Referenzen

References
1 Bonnar J. Blood coagulation and fibrinolysis in obstetrics. Clin Haematol 1973; 2: 213-233
2 Gilabert J, Aznar J, Parilla JJ, Regañon E, Vila V, Estelles A. Alterations in the coagulation and fibrinolysis system in pregnancy, labour and puerperium, with special reference to a possible transitory state of intravascular coagulation during labour. Thromb Haemostas 1978; 40: 387-396
3 Bjørklid E, Storm E, Prydz H. The protein component of human brain thromboplastin. Biochem Biophys Res Commun 1973; 55: 969-976
4 Bjørklid E, Storm E. Purification and some properties of the protein component of tissue thromboplastin from human brain. Biochem J 1977; 165: 89-96
5 Schneider CL. The active principle of placental toxin: Thromboplastin: its inactivator in blood: Antithromboplastin. Am J Physiol 1947; 149: 123-129
6 Nilsen PA. Hypofibrinogenemia in premature separation of the placenta. Acta Obstet Gynecol Scand 1963; 42 (Suppl. 00) 96
7 Dalaker K, Kaplun A, Lyberg T, Prydz H. Synthesis of thromboplastin (Factor III) in mouse placental cells in vitro. Gynecol Obstet Invest 1983; 15: 351-361
8 Dalaker K, Prydz H. Regulation of thromboplastin synthesis in mouse placental cells in vitro. Thromb Haemostas 1983; 50: 831-834
9 Hvatum M, Prydz H. Studies on tissue thromboplastin. I. Solubilization with sodium deoxycholate. Biochem Biophys Acta 1966; 130: 92-101
10 Hjort PE. Intermediate reactions in the coagulation of blood with tissue thromboplastin. Scand J Clin Lab Invest 1957; 9 (Suppl. 27) 183
11 Markwell MAK, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem 1978; 87: 206-210
12 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193: 265-275
13 Torjesen PA, Aakvaag A. The affinity and capacity of the LH receptor of the superluteinized rat ovary in relation to progesterone production. Eur J Obstet Gynecol Reprod Biol 1976; 6: 181-184
14 Hetland Ø, Prydz H. Phospholipase C from Bacillus cereus has sphingomyelinase activity. Scand J clin Lab Invest 1982; 42: 57-61
15 Yam LT, Li CY, Crosby WA. Cytochemical identification of monocytes and granulocytes. Am J clin Path 1971; 55: 283-290
16 Culling CFA. Handbook of Histopathological and Histochemical Techniques. 3.. 267-268 Butterworth, London: 1974
17 Østerud B, Eskeland T. The mandatory role of complement in the endotoxin-induced synthesis of tissue thromboplastin in blood monocytes. FEBS Lett 1982; 149: 75-79
18 Prydz H, Lyberg T, Deteix P, Allison AC. In vitro stimulation of tissue thromboplastin (Factor III) synthesis in human monocytes by immune complexes and lectins. Thromb Res 1978; 15: 465-474
19 Lyberg T, Prydz H. Is lymphocyte cooperation necessary for thromboplastin synthesis by human monocytes?. Clin exp Immunol 1983; 53: 731-738
20 Rothberger H, Lee TK, Deane J, Zimmerman TS. Increase of leukocyte tissue factor activity stimulated by red cells sensitized with human blood group alloantibodies. Thromb Res 1982; 27: 537-547
21 Johnsen ULH, Lyberg T, Galdal KS, Prydz H. Platelets stimulate thromboplastin synthesis in human endothelial cells. Thromb Haemostas 1983; 49: 69-72
22 Østerud B, Flægstad T. Increased tissue thromboplastin activity in monocytes of patients with meningococcal infection related to an unfavourable prognosis. Thromb Haemostas 1983; 49: 5-7
23 Prydz H, Allison AC. Tissue thromboplastin activity of isolated human monocytes. Thromb Haemostas 1978; 39: 582-591
24 Lyberg T, Prydz H. Lectin stimulation of tissue thromboplastin activity in human monocytes in vitro. Thromb Haemostas 1980; 42: 1574-1579
25 Lyberg T, Amlie E, Kaplun A, Prydz H. Macrophage heterogeneity in the thromboplastin response. Scand J Immunol 1983; 18: 235-240
26 Lyberg T, Hetland Ø, Prydz H. Synthesis of thromboplastin protein by a murine macrophage-like cell line. Thromb Haemostas 1982; 47: 154-156
27 Lyberg T, Nilsson K, Prydz H. Synthesis of thromboplastin by U-937 cells. Br J Haematol 1982; 51: 631-641
28 Lyberg T, Galdal KS, Evensen SA, Prydz H. Cellular cooperation in endothelial cell thromboplastin synthesis. Br J Haematol 1983; 53: 85-95
29 Dean RT, Prydz H. Amines induce increased thromboplastin activity in human monocytes. Eur J Biochem 1983; 131: 655-658