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Thromb Haemost 1984; 51(03): 388-391
DOI: 10.1055/s-0038-1661108
Original Article
Schattauer GmbH Stuttgart

Immunocytochemical Localization of Albumin and Factor XIII in Thin Cryo Sections of Human Blood Platelets

Jan J Sixma
1   The Dept. of Haematology, State University Utrecht, The Netherlands
,
Agnes van den Berg
2   The Center for Electron Microscopy, State University Utrecht, The Netherlands
,
Marion Schiphorst
1   The Dept. of Haematology, State University Utrecht, The Netherlands
,
Hans J Geuze
2   The Center for Electron Microscopy, State University Utrecht, The Netherlands
,
Jan McDonagh
3   The Dept. of Pathology, Harvard Medical School and Beth Israel Hospital, Boston, Mass., U.S.A.
› Author Affiliations
Further Information

Publication History

Received 20 February 1984

Accepted 09 April 1984

Publication Date:
19 July 2018 (online)

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Summary

Albumin and factor XIIIa2 were localized in human blood platelets by immunofluorescence and protein A-colloidal gold immuno-electron microscopy using thin and ultrathin cryosections of mildly fixed human blood platelets. Albumin was found in α-granules, co-distributing with β-thromboglobulin in double label experiments. In platelets directly fixed in plasma, without previous washing, albumin was also localized on the plasma membrane and in the surface connecting system. Factor XIIIa2 was localized diffusely through the entire cytoplasmic matrix.

Keywords

Albumin - Factor XIII - α-granules - Immuno-electron microscopy
 

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