Changes in Plasminogen Activator Activity and Plasmin Inhibition in the Pig During Experimental Hypovolaemia

A Smokovitis; M Wagner; M Starlinger; A Opitz; B R Binder

doi:10.1055/s-0038-1661252

Thrombosis and Haemostasis, Table of Contents

Thromb Haemost 1985; 53(01): 130-133
DOI: 10.1055/s-0038-1661252

Original Article

Schattauer GmbH Stuttgart

Changes in Plasminogen Activator Activity and Plasmin Inhibition in the Pig During Experimental Hypovolaemia

A Smokovitis

¹The Department of Physiology, Veterinary Faculty, Aristotelian University of Thessaloniki, Greece

,

M Wagner

²The First Department of Traumatology, Medical School, University of Vienna, Austria

,

M Starlinger

³The First Department of Surgery, Medical School, University of Vienna, Austria

,

A Opitz

²The First Department of Traumatology, Medical School, University of Vienna, Austria

,

B R Binder

⁴The Department of Medical Physiology, Medical School, University of Vienna, Austria

› Author Affiliations

Abstract

PDF Download

Summary

A markedly decreased plasminogen activator activity (PAA) was seen in lung, heart and kidney after experimental hypovolemia in the pig. In 2 of 12 cases, an increased PAA was found in liver and spleen. In skeletal muscle, skin, sciatic nerve and in 10 of 12 cases in liver and spleen PAA was unchanged. A slightly increased plasmin inhibition was noted only in the lung. These changes in tissue PAA were reflected by an increase in the PAA of euglobulin fractions concomitant with a decrease in antiplasmin activity in the blood samples obtained during the bleeding experiments.

Key words

Haemorrhagic hypotension - Fibrinolysis - Plasminogen activator activity - Plasmin inhibition - Pig

PDF (527 kb)

References

References
1 Hardaway RM, Johnson DG. Influence of fibrinolysin on shock. JAMA 1963; 183: 597-599
2 Kottinen YP. Fibrinolysis: chemistry, physiology, pathology, and clinics. Oy Star Ab; Finland: 1968. p 330
3 Büsing CM, Bleyl U. Plasminogen activator activity of pulmonary vessels in shock. Thromb Res 1977; 11: 285-295
4 Rammer L, Saldeen T. Inhibition of fibrinolysis in post-traumatic death. Thrombos Diathes Haemorrh 1970; 24: 68-75
5 Risberg B, Peterson HI. Pulmonary tissue fibrinolysis after drum trauma. An experimental study in the rat Circ Shock 1976; 3: 153-159
6 Brakman P, Albrechtsen OK, Astrup T. A comparative study of coagulation and fibrinolysis in blood from normal men and women. Br J Haematol 1966; 12: 74-85
7 Binder BR, Spragg J, Austen KF. Purification and characterization of human vascular plasminogen activator derived from blood vessel perfusates. J Biol Chem 1979; 254: 1998-2003
8 Teger-Nilsson A-C, Friberger P, Gyzander E. Determination of a new rapid antiplasmin in human blood by using a plasmin specific tripeptide substrate. Scand J Clin Lab Invest 1977; 37: 403-409
9 Smokovitis A. Normal liver actually possesses a high vascular plasminogen activator activity. Experientia 1979; 35: 776-777
10 Smokovitis A. Comparative histochemical studies on fibrinolysis in whole organs and isolated tissue layers. Thromb Haemostas 1979; 42: 909-923
11 Smokovitis A, Maier M, Binder BR. Comparison between vascular plasminogen activator activity and changes in blood flow in the renal cortex in pigs. Thromb Haemostas 1984; 51: 150-153
12 Hoylaerts M, Rijken DC, Lijnen HR, Collen D. Kinetics of the activation of plasminogen by human tissue plasminogen activator. Role of fibrin J Biol Chem 1982; 257: 2912-2919
13 Wiman B, Chmielewska J, Ranby M. Inactivation of tissue plasminogen activator in plasma. Demonstration of a complex with a new rapid inhibitor J Biol Chem 1984; 259: 3644-3647
14 Loskutoff DJ, Van Mourik JA, Erickson LA, Lawrence D. Detection of an usually stable fibrinolytic inhibitor produced by bovine endothelial cells. Proc Natl Acad Sci 1983; 80: 2956
15 Rothe CF. Heart failure and fluid loss in hemorrhagic shock. Fed Proc 1970; 29: 1854-1860
16 Altura BM, Halevy S. Beneficial and detrimental actions of histamine H₁- and H₂-receptor antagonists in circulatory shock. Proc Natl Acad Sci USA 1978; 75: 2941-2944
17 Famebo LO, Hallman H, Hamberger B, Jonsson G. Catecholamines and haemorrhagic shock in awake und anesthetized rats. Circ Shock 1979; 6: 109-118
18 McNeill JR, Stark RD, Greenway CV. Intestinal vasoconstriction after haemorrhage: roles of vasopressin and angiotensin. Am J Physiol 1970; 219: 1342-1347
19 Selkurt EE. Status of investigative aspects of hemorrhagic shock. Fed Proc 1970; 29: 1832-1835
20 Trachte GJ, Lefer AM. Effect of angiotensin II receptor blockade by (Sar¹-Ala⁸) angiotensin II in hemorrhagic shock. Am J Physiol 1979; 236: H280-H285
21 Lefer AM. Properties of cardioinhibitory factors produced in shock. Fed Proc 1978; 37: 2734-2740
22 Ali SY, Lack CH. Studies on the tissue activator of plasminogen. Biochem J 1965; 96: 63-74
23 Beard EL, Montouri MH, Danos GJ, Busuttil RW. Plasminogen activator activity of rat lysosomes. Proc Soc Exp Biol Med 1968; 129: 804-808
24 Beard EL, Busuttil RW, Gottshalk SK. Stress induced release of plasminogen activator from lysosomes. Thrombos Diathes Haemorrh 1969; 21: 20-25
25 Lack CH, Ali SY. Tissue activator of plasminogen. Nature 1964; 201: 1030-1031
26 Holemans R. Enhancement of fibrinolysis in the dog by injection of vasoactive drugs. Am J Physiol 1965; 208: 511-520
27 Bernik MB, Oller EP. Increased plasminogen activator (urokinase) in tissue culture after fibrin deposition. J Clin Invest 1973; 52: 823-834
28 McNicol GP, Douglas AS. The fibrinolytic enzyme system. In Human blood coagulation, haemostasis and thrombosis. Biggs R. (Ed) Blackwell Scientific Publ; London: 1976. p 414
29 Carriere S, Thorbum GD, O’Morchoe CC C, Barger AC. Intrarenal distribution of blood flow in dogs during haemorrhagic hypotension. Circ Res 1966; 19: 167-179
30 Passmore JC, Leffler CW, Neiberger RE. A critical analysis of renal blood flow distribution during hemorrhage in dogs. Circ Shock 1978; 5: 327-338
31 Rector JB, Stein JH, Bay WH, Osgood RW, Ferris TF. Effect of haemorrhage and vasopressor agents on distribution of renal blood flow. Am J Physiol 1972; 222: 1125-1131
32 Grega GJ, Daugherty Jr RM, Scott JB, Radawski DP, Haddy FJ. Effect of pressure, flow, and vasoactive agents on vascular resistance and capillary filtration in canine fetal, newborn, and adult lung. Microvasc Res 1971; 3: 297-307
33 Wiegman DL, Harris PD, Miller FN, Morff RJ, Wilmoth FR. Survival and microvascular responses to hemorrhage with three anesthetic combinations. Am J Physiol 1978; 235: H753-H758
34 Rosing DR, Redwood DR, Brakman P, Astrup T, Epstein SE. The fibrinolytic response of man to vasoactive drugs measured in arterial blood. Thromb Res 1978; 13: 419-428
35 Jedrychowski A, Parbhoo SP, Hillenbrand P. Plasminogen activator in bile during extracorporeal perfusion of pig liver. Gut 1972; 13: 54-57
36 Losito R, Lemieux E. Streptokinase in the isolated perfused liver. Am J Physiol 1974; 227: 477-481
37 Losito R, Gattiker H, Lemieux E, Longre B. Comparative effects of an erythrocyte enriched synthetic replenisher and whole blood on streptokinase activity in the isolated perfused liver. Thromb Res 1979; 14: 125-130
38 Habal FM, Burrowes CE, Movat HZ. Generation of kinin by plasma kallikrein and plasmin and the effect of alpha-l-antitrypsin and antithrombin III on the kininogenases. In Kinins, Pharmacodynamics and Biological Roles. Sicuteri F, Back N, Haberland GL. (Eds) Plenum Press; New York: 1976. p 23
39 Stormorken H. Relation of the fibrinolytic to other biological systems. Thrombos Diathes Haemorrh 1975; 34: 378-385
40 Osmond DH, Lo EK, Loh AY, Zingg EA, Hedlin AH. Kallikrein and plasmin as activators of inactive renin. Lancet 1978; 2: 1375-1376
41 Bagge L, Saldeen T. The primary fibrinolysis inhibitor and trauma. Thromb Res 1978; 13: 1131-1136
42 Bagge L, Carlin G, Hjelmstedt A, Högstorp H, Jakobsson H, Modig J, Saldeen T. Fibrinolysis inhibition after a standardized trauma, total hip replacement surgery. Thromb Haemostas 1979; 42: 278 (Abstr.)
43 Bergentz SE, Nilsson IM. Effect of trauma on coagulation and fibrinolysis in dogs. Acta Chir Scand 1961; 122: 21-29
44 Lindquist O, Bagge L, Saldeen T. Induction of endogenous fibrinolysis inhibition in the dog. Acta Chir Scand 1976; 142: 20-25