Thrombosis and Haemostasis, Table of Contents Thromb Haemost 1986; 56(03): 260-262DOI: 10.1055/s-0038-1661662 Original Article Schattauer GmbH Stuttgart Interaction of Vasopressin with Human Blood Platelets: Dependency on Mg2+ Isabella Roos The Department of Research, Kantonsspital Basel, Switzerland , Fabrizia Ferracin The Department of Research, Kantonsspital Basel, Switzerland , Alfred Pletscher The Department of Research, Kantonsspital Basel, Switzerland › Author AffiliationsRecommend Article Abstract PDF Download Buy Article Summary Arginine-vasopressin (AVP) in the presence of Mg2+ but not in the absence of bivalent cations led to accumulation of [32P]-phosphatidic acid ([32P]-PA) in human blood platelets. Mg2+ also enhanced the specific binding of [3H]-AVP to intact platelets. The concentrations of the cation which enabled AVP to cause half maximal rise of [32P]-PA and those inducing half maximal [3H]-AVP-binding were of the same order. It is concluded that the stimulation of phosphatidyl inositide breakdown by AVP in presence of Mg2+ is at least partially due to a Mg2+-induced enhancement of specific AVP-binding to the platelet membranes. Key words Key wordsBlood platelets of man - Arginine-vasopressin - Phos-phoinositide turnover: dependence on Mg2+ - Magnesium ions - Vasopressin binding: dependence on Mg2+ PDF (1926 kb) References References 1 Siess W, Stifel M, Binder H, Weber PC. Activation of Vrreceptors by vasopressin stimulates inositol phospholipid hydrolysis and arachido-nate metabolism in human platelets. Biochem J 1986; 233: 83-91 2 Pollock WK, MacIntyre DE. Desensitization and antagonism of vasopressin-induced phosphoinositide metabolism and elevation of cytosolic free calcium concentration in human platelets. Biochem J 1986; 234: 67-73 3 Erne P, Pletscher A. Vasopressin-induced activation of human blood platelets: prominent role of Mg2+ . Naunyn-Schmiedeberg’s Arch Pharmacol 1985; 329: 97-99 4 Pletscher A, Erne P, Bürgisser E, Ferracin F. Activation of human blood platelets by arginine-vasopressin. Role of bivalent cations. Mol Pharmacol 1985; 28: 508-514 5 Berrettini WH, Post RM, Worthington EK, Casper JB. Human platelet vasopressin receptors. Life Sci 1982; 30: 425-432 6 Lapetina EG, Cuatrecasas P. Stimulation of phosphatidic acid production in platelets precedes the formation of arachidonate and parallels the release of serotonin. Biochim Biophys Acta 1979; 573: 394-402 7 Laubscher A, Pletscher A. Shape change and uptake of 5-hydroxy-tryptamine in human blood platelets: Action of neuropsychotropic drugs. Life Sci 1979; 24: 1833-1840 8 Michell RH. Inositol phospholipids and cell surface receptor function. Biochim Biophys Acta 1975; 415: 81-87 9 Lapetina EG. Metabolism of inositides and the activation of platelets. Life Sci 1983; 32: 2069-2082 10 Jolles J, Zwiers H, Dekker A, Wirtz KW A, Gispen WH. Corticotropin-(1-24)tetracosapeptide affects protein phosphorylation and polyphosphoinositide metabolism in rat brain. Biochem J 1981; 194: 283-291 11 Rittenhouse SE. Inositol lipid metabolism in the response of stimulated platelets. Cell Calcium 1982; 3: 311-322 12 Berridge MJ. Inositol triphosphate and diacylglycerol as second messengers. Biochem J 1984; 220: 345-360 13 Berridge MJ, Irvine RF. Inositol triphosphate, a novel second messenger in cellular transduction. Nature 1984; 312: 315-321
