Verapamil Inhibits Platelet Aggregation by a Calcium-Independent Mechanism

Summary

We studied the inhibitory effects of the calcium channel blocker verapamil both on platelet aggregation and intracellular calcium [Ca²⁺]_i in platelets loaded with a fluorescent indicator (quin 2).

The inhibitory effects of verapamil on the platelet aggregation response to both thrombin and ionomycin were seen to be clearly dissociated from the verapamil-induced inhibition of the [Ca²⁺]_i increase produced by these agonists. Verapamil-induced inhibition of platelet aggregation was also obtained when using the “calcium-independent” agonist phorbol-myristate acetate (PMA). It may be deduced that a calcium-independent mechanism plays a role in verapamil-induced inhibition of platelet aggregation. We postulate that this mechanism may operate via a protein-kinase C pathway.

Key words

Verapamil - Intracellular calcium - Platelet aggregation - Thrombin - Ionomycin

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