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Thromb Haemost 1983; 50(02): 530-533
DOI: 10.1055/s-0038-1665247
Original Article
Schattauer GmbH Stuttgart

Functional and Antigenic Antithrombin III Values in Plasma and Serum from 115 Regular Blood Donors and Antithrombin III In Prediction of Postoperative Thrombosis

Autor*innen

  • D W T Nilsen

    The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

  • M Jeremic

    The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

  • O K Weisert

    The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

  • E Fønstelien

    The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway

  • H C Godal

    The State Laboratory of Microbiology, Department of Immunohaematology and Blood Transfusion, Lillehammer, the Haematological Research Laboratory and the Laboratory of Clinical Physiology, Ullevål University Clinic, Oslo, Norway
Weitere Informationen

Publikationsverlauf

Received 04. Januar 1983

Accepted 18. Mai 1983

Publikationsdatum:
18. Juli 2018 (online)

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Summary

Antithrombin III (AT-III) was determined functionally using chromogenic substrate (S-2238) and immunologically using radial immunodiffusion (RID) in plasma and serum from 115 blood donors. There was a decrease in functional activity by 43.5% and in antigen concentration by 18.4% during in vitro coagulation when corresponding serum and plasma samples were compared. A positive correlation was found between the two methods in plasma (r=0.784) as well as in serum (r = 0.658). RID in serum correlated well with RID in plasma (r = 0.811), but the correlation for S-2238 in plasma and serum was poor (r = 0.411). Functional AT-III decrease during coagulation was uncorrelated with age and fibrinogen, and was not statistically affected by sex, smoking or blood groups.

Preoperative functional AT-III activity was measured in 25 patients undergoing elective hip replacement, 60% of whom developed postoperative thrombosis. The functional activity in serum discriminated well between the thrombotic and nonthrombotic group of patients (p<0.025), whereas the activity in plasma showed only a minor difference.

Keywords

Plasma-antithrombin III - Serum-antithrombin III - Post-operative thrombosis