Effects of Superoxide Dismutase and Catalase on Disseminated Intravascular Coagulation In Rats

Summary

The effects of superoxide dismutase (SOD) and catalase on endotoxin-induced experimental disseminated intravascular coagulation (DIC) were studied in rats. Experimental DIC was induced by a 4 hr sustained infusion of endotoxin at a dose of 100 mg/kg. The rats were subcutaneously injected with SOD at 0.5, 5.0 or 50.0 mg/kg, or catalase at 0.01, 0.1 or 1.0 mg/kg, followed by the continuously infusion of 100 mg/kg/4hr of endotoxin. A preventive effect against DIC was noted in all the parameters, such as fibrinogen and fibrin degradation products, fibrinogen level, prothrombin time, partial thromboplastin time, platelet count and the number of renal glomeruli with fibrin thrombi, in the rats treated with 50.0 mg/kg of SOD or 1.0 mg/kg of catalase. When 50.0 mg/kg of SOD or 1.0 mg/kg of catalse was injected subcutaneously at 1, 2 or 3 hr after the initiation of the endotoxin-infusion, the protective effect against DIC was noted in all the parameters.

Keywords

Disseminated intravascular coagulation - Endotoxin - Superoxide dismutase - Catalase - Rat

• References

• 1 Fridovich I. The biology of oxygen radicals. Science 1978; 201: 875-880
• 2 Fridovich I. Superoxide dismutase. Ann Rev Biochem 1975; 44: 147-159
• 3 Sies H. Biochemie des Peroxysoms in der Leberzelle. Angew Chem 1974; 86: 789-801
• 4 Handin RI, Carabin R, Boxer GJ. Enhancement of platelet function by superoxide anion. J Clin Invest 1977; 59: 959-965
• 5 Higashi O, Kikuchi Y. Effects of vitamin E on the aggregation and the lipid peroxidation of platelets exposed to hydrogen peroxide. Tohoku J Exp Med 1974; 112: 271-278
• 6 Beller FK, Graeff H. Deposition of glomerular fibrin in the rabbit after infusion with endotoxin. Nature 1967; 215: 295-296
• 7 Yoshikawa T, Furukawa Y, Murakami M, Takemura S, Kondo M. Experimental model of disseminated intravascular coagulation induced by sustained infusion of endotoxin. Res Exp Med 1981; 179: 223-228
• 8 Ferreira HC, Murat LG. Immunological method for demonstrating fibrin degradation products in serum and its use the diagnosis of fibrinolytic states. Br J Haematol 1963; 9: 299-310
• 9 Ratnoff OD, Menzie C. A new method for the determination of fibrinogen in small samples of plasma. J Lab Clin Med 1951; 37: 316-320
• 10 Quick AJ, Stanly BM, Bancroft FW. A study of the coagulation defect in hemophilic and in jaundice. Am J Med Sci 1935; 190: 501-511
• 11 Langdeil RD, Wagner RH, Brinkhous KM. Effect of antihemophilic factor on one-stage clotting time. J Lab Clin Med 1953; 47: 637-647
• 12 Brecker G, Cronkite EP. Morphology and enumeration of human blood platelets. J Appl Physiol 1950; 3: 365-377
• 13 Weigert C. Uber eine neue Methode zur Färbung von Fibrin und von Microorganismen. Fortschr Med 1887; 5: 228-232
• 14 Yoshikawa T, Murakami M, Furukawa Y, Kato H, Takemura S, Kondo M. Lipid peroxidation and experimental disseminated intravascular coagulation in rats induced by endotoxin. Thromb Haemostas 1983; 49: 214-216
• 15 Yoshikawa T, Furukawa Y, Murakami M, Watanabe K, Kondo M. Effect of vitamin E on endotoxin-induced disseminated intravascular coagulation in rats. Thromb Haemostas 1982; 48: 235-237
• 16 Steiner M, Anastasi J, Vitamin E. An inhibitor of the platelet release reaction. J Clin Invest 1976; 57: 732-737
• 17 Panganamala RV, Miller JS, Gwebu ET, Sharma HM, Cornwell DG. Differential inhibitory effects of vitamin E and other antioxidants on prostaglandin synthetase, platelet aggregation and lipoxidase. Prostaglandins 1977; 14: 261-271
• 18 White JG, Rao GH, Gerrard JM. Effects of nitroblue tetrazolium and vitamin E on platelet ultrastructure aggregation, and secretion. Am J Pathol 1977; 88: 387-402
• 19 Greenberg M, Grady RW, Peterson CM. Inhibition of platelet function with 2,3-dihydroxybenzoic acid. Br J Haematol 1977; 37: 569-577
• 20 Kellogg IIIE W, Fridovich I. Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J Biol Chem 1975; 250: 8812-8817
• 21 Goldberg B, Stern A. Superoxide anion as a mediator of drug-induced oxidative hemolysis. J Biol Chem 1976; 251: 6468-6470
• 22 Kellogg IIIE W, Fridovich I. Liposome oxidation and erythrocyte lysis by enzymically generated superoxide and hydrogen peroxide. J Biol Chem 1977; 252: 6721-6728
• 23 Michelson AM, Buckingham ME. Effects of superoxide radicals on myoblast growth and differentiation. Biochem Biophys Res Commun 1974; 58: 1079-1086
• 24 Tappel AL. Lipid peroxidation damage to cell components. Fed Proc 1973; 32: 1870-1874
• 25 Minna JD, Robboy SI, Colman RW. Disseminated intravascular coagulation in man. Charles ThomasC. (Eds) Springfield; 1974 pp 3-18