Geburtshilfe Frauenheilkd 2018; 78(10): 178
DOI: 10.1055/s-0038-1671290
Poster
Freitag, 02.11.2018
Endokrinologie und Reproduktionsmedizin II
Georg Thieme Verlag KG Stuttgart · New York

Effects of different progestins on prostaglandin biosynthesis in human endometrial explants

K Roth
1  University Medical Center Freiburg, Department of Obstetrics and Gynecology, Freiburg, Deutschland
,
HP Zahradnik
1  University Medical Center Freiburg, Department of Obstetrics and Gynecology, Freiburg, Deutschland
,
W Schäfer
1  University Medical Center Freiburg, Department of Obstetrics and Gynecology, Freiburg, Deutschland
› Author Affiliations
Further Information

Publication History

Publication Date:
20 September 2018 (online)

 

Objective:

To compare the effects of chlormadinone acetate (CMA), dienogest (DNG) and drospirenone (DRSP) on prostaglandin biosynthesis in a human endometrial explants model.

Study Design:

Human endometrial explants obtained by aspiration curettage and human endometrial YHES cells were stimulated with interleukin-1ß (IL-1ß) and exposed to CMA, DNG, DRSP or dexamethasone (DEX; YHES cells). Cellular messenger RNA (mRNA) levels of cyclooxygenase-2 (COX-2) were analyzed by reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR). Concentrations of prostaglandin F(PGF) in culture supernatants were measured by ELISA.

Results:

CMA exerted after IL-1ß-stimulation a stronger downregulation of COX-2 mRNA compared to DNG and DRSP in human explants (-55% vs. -40% and 46%, respectively). The effect of CMA on COX-2 mRNA was significantly stronger (p = 0.025) than that of DNG. Moreover, the effect of CMA was independent from cycle phase or presence of endometriosis. In order to evaluate the impact of the investigated progestins on effector molecules, PGFrelease was determined in supernatants. Again, CMA reduced the PGF release significantly by an average of -60% (p < 0.01). In contrast, no significant reduction was found for DNG and DRSP. In YHES cells, only DEX but not the progestins under study exerted a significant down-regulating effect (-79%, p < 0.01) on COX-2 mRNA after IL-1ßstimulation.

Conclusion:

Among the tested progestins CMA displayed the most consistent suppression of prostaglandin biosynthesis in human endometrial explants.