Thromb Haemost 2019; 119(05): 744-757
DOI: 10.1055/s-0039-1679908
Cellular Haemostasis and Platelets
Georg Thieme Verlag KG Stuttgart · New York

Epithelial (E)-Cadherin is a Novel Mediator of Platelet Aggregation and Clot Stability

Vanessa M. Scanlon*
1  Department of Laboratory Medicine, Yale University, New Haven, Connecticut, United States
2  The Yale Stem Cell Center, Yale University, New Haven, Connecticut, United States
,
Alexandra M. Teixeira*
3  Department of Pathology, Yale University, New Haven, Connecticut, United States
,
Tarun Tyagi
4  Department of Internal Medicine, Yale University, New Haven, Connecticut, United States
,
Siying Zou
5  Department of Cell Biology, Yale University, New Haven, Connecticut, United States
,
Ping-Xia Zhang
1  Department of Laboratory Medicine, Yale University, New Haven, Connecticut, United States
2  The Yale Stem Cell Center, Yale University, New Haven, Connecticut, United States
,
Carmen Jane Booth
6  Department of Comparative Medicine, Yale University, New Haven, Connecticut, United States
,
M. Anna Kowalska
7  Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
8  Institute of Medical Biology, Polish Academy of Sciences, Philadelphia, Pennsylvania, United States
,
Jialing Bao
9  Department of Pathology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
10  Department of Laboratory Medicine, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
11  University of Pennsylvania, Philadelphia, Pennsylvania, United States
,
John Hwa
4  Department of Internal Medicine, Yale University, New Haven, Connecticut, United States
,
Vincent Hayes
12  Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
,
9  Department of Pathology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
10  Department of Laboratory Medicine, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
11  University of Pennsylvania, Philadelphia, Pennsylvania, United States
,
Mortimer Poncz
11  University of Pennsylvania, Philadelphia, Pennsylvania, United States
12  Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, United States
,
Diane S. Krause
1  Department of Laboratory Medicine, Yale University, New Haven, Connecticut, United States
2  The Yale Stem Cell Center, Yale University, New Haven, Connecticut, United States
3  Department of Pathology, Yale University, New Haven, Connecticut, United States
5  Department of Cell Biology, Yale University, New Haven, Connecticut, United States
› Author Affiliations
Funding These studies were supported by NIH R01 DK094934, the Yale NIDDK-funded Cooperative Center of Excellence in Hematology (U54 DK106857), R01 DK086267, T32HL007974 and R01 HL121323.
Further Information

Publication History

18 May 2018

10 January 2019

Publication Date:
12 March 2019 (eFirst)

Abstract

Cadherins play a major role in mediating cell–cell adhesion, which shares many parallels with platelet–platelet interactions during aggregate formation and clot stabilization. Platelets express epithelial (E)-cadherin, but its contribution to platelet function and/or platelet production is currently unknown. To assess the role of E-cadherin in platelet production and function in vitro and in vivo, we utilized a megakaryocyte-specific E-cadherin knockout mouse model. Loss of E-cadherin in megakaryocytes does not affect megakaryocyte maturation, platelet number or size. However, platelet dysfunction in the absence of E-cadherin is revealed when conditional knockout mice are challenged with acute antibody-mediated platelet depletion. Unlike wild-type mice that recover fully, knockout mice die within 72 hours post-antibody administration, likely from haemorrhage. Furthermore, conditional knockout mice have prolonged tail bleeding times, unstable clot formation, reduced clot retraction and reduced fibrin deposition in in vivo injury models. Murine platelet aggregation in vitro in response to thrombin and thrombin receptor activating peptide is compromised in E-cadherin null platelets, while aggregation in response to adenosine diphosphate (ADP) is not significantly different. Consistent with this, in vitro aggregation of primary human platelets in response to thrombin is decreased by an inhibitory E-cadherin antibody. Integrin activation and granule secretion in response to ADP and thrombin are not affected in E-cadherin null platelets, but Akt and glycogen synthase kinase 3β (GSK3β) activation are attenuated, suggesting a that E-cadherin contributes to aggregation, clot stabilization and retraction that is mediated by phosphoinositide 3-kinase/Akt/GSK3β signalling. In summary, E-cadherin plays a salient role in platelet aggregation and clot stability.

* These authors contributed equally to this work.