Abstract
Objectives Inner carious dentin is specified with decreased minerals and collagen cross-links
but without protein denaturation. Current minimally invasive dentistry concepts recommend
removal of only the outer layer of carious dentin and biomodification of repairable
inner carious dentin. The present study aims to investigate the possibility of functional
repair of this layer using silver diamine fluoride (SDF) and grape seed extract (GSE).
Materials and Methods Molar teeth with occlusal caries were used to prepare caries-affected dentin specimens
for hardness and elastic modulus measurements. The specimens of each test were divided
randomly into four equal groups. In the GSE group, the specimens were immersed in
6.5% GSE solution for 10 minutes. In the SDF group, the specimens underwent a topical
application of a 30% SDF. In the GSE+SDF group, first the specimens were immersed
in GSE and then exposed to SDF. In the SDF+GSE group, first SDF was applied and then
the specimens were immersed in GSE. Microhardness measurements were taken at baseline
and after treatment. A control group with distilled water treatment was also prepared
for elastic modulus measurements.
Statistical Analysis One-way analysis of variance and post-hoc tests were used for statistical analysis.
Results There were significant differences in H1-H0 (final hardness—baseline hardness) among the groups. Baseline and final hardness
of each group was also significantly different (SDF>SDF+GSE>GSE>GSE+SDF). Elastic
modulus of SDF and SDF+GSE increased compared to the control group.
Conclusions SDF and SDF+GSE treatment can be recommended to increase hardness and elastic modulus
of caries-affected dentin.
Keywords
silver diamine fluoride - grape seed extract - Knoop hardness - modulus of elasticity
- caries-affected dentin
