Potential of Distilled Liquid Smoke Derived from Coconut (Cocos nucifera L) Shell for Traumatic Ulcer Healing in Diabetic Rats

Abstract

Objective Distilled liquid smoke (DLS) is a result of coconut processing by-product that not only serves as a natural food preservative but also has a promising therapeutic effect. The healing potential of DLS derived from coconut (Cocos nucifera L) shell was investigated on a traumatic ulcer with the diabetic rat.

Materials and Methods DLS was analyzed the component by gas chromatograph mass spectrometry. Diabetic condition was induced by alloxan in 55 male Wistar rats. Ten mm of traumatic ulcer was made along the labial fornix incisive inferior after the diabetic condition was confirmed. Then DLS coconut shell, benzydamine hydrochloride, and sterile distilled water were applied topically for 3, 5, and 7 days. The potential healing was evaluated based on the expression of nuclear factor kappa beta (NFκB) and tumor necrosis factor alpha (TNF-α) on macrophages using immunohistochemical staining and the amount of collagen using Masson Trichome staining. The difference between each group was analyzed using one-way analysis of variance. The least significant difference test is used to determine the significant difference (p < 0.05).

Results The major compounds found were phenol (36.6%), 2-methoxyphenol (guaiacol) (25.2%), furfural (17.8%), and 4-ethyl-2-methoxyphenol (3.5%) with 28 other minor constituents. The lowest NFκB and TNF-α expression on macrophage was observed by topical application of DLS derived from coconut shell for 3, 5, and 7 days of treatment. The amount of collagen was increased and indicated by the highest result of DLS compared to others.

Conclusion The DLS derived from coconut (Cocos nucifera L) shell was able to improve traumatic ulcer healing in a person with diabetes.

Keywords:

coconut shell - diabetic - distilled liquid smoke - traumatic ulcer healing

• References

• 1 Wagiman FX, Ardiansyah A. Witjaksono. Activity of coconut-shell liquid-smoke as an insecticide on the rice brown planthopper (Nilaparvata lugens). J Agric Biol Sci 2014; 9 (09) 293-296
• 2 Zuraida I, Sukarno. Budijanto S. Antibacterial activity of coconut shell liquid smoke (CS-LS) and its application on fish ball preservation. Int Food Res J 2011; 18: 405-410
• 3 Achmadi SS, Mubarik NR, Nursyamsi R, Septiaji P. Characterization of redistilled liquid smoke of oil-palm shells and its application as fish preservatives. J Appl Sci (Faisalabad) 2013; 13 (03) 401-408
• 4 Yusnaini S, Soeparno S, Suryanto E, Armunanto R. Physical, chemical and sensory properties of Kenari (Canariun indicum L.) shell liquid smoke-immersed-beef on different level of dilution. J Indones Trop Anim Agric 2012; 37 (01) 27-33
• 5 Kim SP, Yang JY, Kang MY, Park JC, Nam SH, Friedman M. Composition of liquid rice hull smoke and anti-inflammatory effects in mice. J Agric Food Chem 2011; 59 (09) 4570-4581
• 6 Lombok JZ, Setiaji B, Trisunaryanti W, Wijaya K. Effect of pyrolysis temperature and distillation on character of coconut shell liquid smoke. Asian J Sci Technol 2014; 5 (06) 320-325
• 7 Purba R, Suseno SH, Izaki AF, Muttaqin S. Application of liquid smoke and chitosan as natural preservatives for tofu and meatballs. Int J Appl Sci Technol 2014; 4 (02) 212-217
• 8 Desniorita M. The effect of adding liquid smoke powder to shelf life of sauce. Int J Adv Sci Eng Inf Technol 2015; 5 (06) 457-459
• 9 Tarawan VM, Mantilidewi KI, Dhini IM, Radhiyanti PT, Sutedja E. Coconut shell liquid smoke promotes burn wound healing. J Evid Based Complementary Altern Med 2016; 38: 1-5
• 10 Anggraini SPA, Yuniningsih S. Liquid smoke purification process for benzo (a) pyrene levels lowering on food safety. J Agric Food Tech 2013; 3 (12) 1-4
• 11 Budaraga IK, Arnim. Marlida Y, Usman B. Characteristics of cinnamon liquid smoke produced using several purification techniques. Am J Food Sci Nutr Res 2016; 3 (02) 16-21
• 12 Budijanto S, Hasbullah R, Prabawati S, Zuraida I. Identifikasi dan Uji Keamanan Asap Cair Tempurung Kelapa untuk Produk Pangan. J Pascapanen 2008; 5 (01) 32-40
• 13 Yang JY, Kang MY, Nam SH, Friedman M. Antidiabetic effects of rice hull smoke extract in alloxan-induced diabetic mice. J Agric Food Chem 2012; 60 (01) 87-94
• 14 Costa G, Francisco V, Lopes MC, Cruz MT, Batista MT. Intracellular signaling pathways modulated by phenolic compounds: application for new anti-inflammatory drugs discovery. Curr Med Chem 2012; 19 (18) 2876-2900
• 15 Meircurius DwiCS, Tantiana T, Arundina I. Analgesic effect of coconut shell (Cocos nucifera L) liquid smoke on mice. Dent J (Majalah Kedokt Gigi) 2012; 45 (03) 156-160
• 16 Tripathi R, Tripathi K. Management of non-healing oral ulcer in diabetic patient using topical application of epidermal growth factor: a case report. Sch Acad J Biosci 2015; 3 (08) 640-643
• 17 Bajaj S, Prasad S, Gupta A, Singh VB. Oral manifestations in type-2 diabetes and related complications. Indian J Endocrinol Metab 2012; 16 (05) 777-779
• 18 Ravindran R, Deepa M, Sruthi A. et al. Evaluation of oral health in type ii diabetes mellitus patients. OMPJ 2015; 6 (01) 525-531
• 19 Xu F, Zhang C, Graves DT. Abnormal cell responses and role of TNF-α in impaired diabetic wound healing. BioMed Res Int 2013; 2013754802: 754802
• 20 Eguia-del ValleA, Martinez-Conde-Llamosas R, López-Vicente J, Uribarri-Etxebarria A, Aguirre-Urizar J-M. Salivary levels of tumour necrosis factor-alpha in patients with recurrent aphthous stomatitis. Med Oral Patol Oral Cir Bucal 2011; 16 (01) e33-e36
• 21 Avci E, Akarslan ZZ, Erten H, Coskun-Cevher S. Oxidative stress and cellular immunity in patients with recurrent aphthous ulcers. Braz J Med Biol Res 2014; 47 (05) 355-360
• 22 Brizeno LA, Assreuy AM, Alves AP. et al. Delayed healing of oral mucosa in a diabetic rat model: implication of TNF-α, IL-1β and FGF-2. Life Sci 2016; 155: 36-47
• 23 Parashar A. Mouthwashes and their use in different oral conditions. Sch J Dent Sci J Dent Sci 2015; 2 (2B) 186-191
• 24 Radenković M, Stojanović M, Prostran M. Experimental diabetes induced by alloxan and streptozotocin: the current state of the art. J Pharmacol Toxicol Methods 2016; 78: 13-31
• 25 Bako HY, Mohammad JS, Waziri PM, Bulus T, Gwarzo MY, Zubairu MM. Lipid profile of alloxan-induced diabetic Wistar rats treated with methanolic extract of Adansonia digitata fruit pulp. Sci World J 2014; 9 (02) 19-24
• 26 Hitomi S, Ono K, Miyano K. et al. Novel methods of applying direct chemical and mechanical stimulation to the oral mucosa for traditional behavioral pain assays in conscious rats. J Neurosci Methods 2015; 239: 162-169
• 27 Kılıç C, Güleç PekerEG, Acartürk F, Kılıçaslan SM, Çoşkun CevherŞ. Investigation of the effects of local glutathione and chitosan administration on incisional oral mucosal wound healing in rabbits. Colloids Surf B Biointerfaces 2013; 112: 499-507
• 28 He S, Atkinson C, Qiao F, Chen X, Tomlinson S. Ketamine-xylazine-acepromazine compared with isoflurane for anesthesia during liver transplantation in rodents. J Am Assoc Lab Anim Sci 2010; 49 (01) 45-51
• 29 Rahayu RP, Prasetyo RA, Purwanto DA, Kresnoadi U, Iskandar RPD, Rubianto M. The immunomodulatory effect of green tea (Camellia sinensis) leaves extract on immunocompromised Wistar rats infected by. Candida albicans. . Vet World 2018; 11 (06) 765-770
• 30 Chawla H, Urs AB, Augustine J. Association of macrophages with angiogenesis in oral epithelial dysplasia, oral verrucous carcinoma, an immunohistochemical study. Appl Immunohistochem Mol Morphol 2015; 25 (03) 203-208
• 31 Mohamad SH. Tumor necrosis factor-alpha (TNF-α) expression in mice infected with aspergillus fumigatus using immunohistochemical technique. IJSBAR 2016; 25 (01) 183-189
• 32 Ali I, Bahaitham H, Naebulharam R. A comprehensive kinetics study of coconut shell waste pyrolysis. Bioresour Technol 2017; 235 March 1-11
• 33 Kan T, Strezov V, Evans TJ. Lignocellulosic biomass pyrolysis: a review of product properties and effects of pyrolysis parameters. Renew Sustain Energy Rev 2016; 57: 1126-1140
• 34 Gao Y, Yang Y, Qin Z, Sun Y. Factors affecting the yield of bio-oil from the pyrolysis of coconut shell. Springerplus 2016; 5 (333) 333
• 35 Roslam WIWN, Hisham MWM, Yarmo MA, Hin TY. A review on bio-oil production from biomass by using pyrolysis method. Renew Sustain Energy Rev 2012; 16 (08) 5910-5923
• 36 Akhtar J, Saidina AminN. A review on operating parameters for optimum liquid oil yield in biomass pyrolysis. Renew Sustain Energy Rev 2012; 16 (07) 5101-5109
• 37 Budaraga IK, Armin. Marlida Y, Bulanin U. Analysis of liquid smoke chemical components with GC MS from different raw materials variation production and pyrolysis temperature level. Int J Chemtech Res 2016; 9 (06) 694-708
• 38 Stefanidis SD, Kalogiannis KG, Iliopoulou EF, Michailof CM, Pilavachi PA, Lappas AA. A study of lignocellulosic biomass pyrolysis via the pyrolysis of cellulose, hemicellulose and lignin. J Anal Appl Pyrolysis 2014; 105: 143-150
• 39 Rout T, Pradhan D, Singh RK, Kumari N. Exhaustive study of products obtained from coconut shell pyrolysis. J Environ Chem Eng 2016; 4 (03) 3696-3705
• 40 Yuniningsih S, Anggraini SPA. Characterization of liquid smoke from coconut shell to be applicated as safe food preservatives for human health. J Agric Food Chem 2013; 3 (02) 1-5
• 41 Hadanu R, Apituley DAN. Volatile compounds detected in coconut shell liquid smoke through pyrolysis at a fractioning temperature of 350-420C. Makara J. Science 2016; 20 (03) 95-100
• 42 Scozzafava A, Passaponti M, Supuran CT, Gulcin I. Carbonic anhydrase inhibitors: guaiacol and catechol derivatives effectively inhibit certain human carbonic anhydrase isoenzymes (hCA I, II, IX, and XII). J Enzyme Inhib Med Chem 2014; 6366: 1-6
• 43 Lauraguais A, Coeur-tourneur C, Cassez A, Deboudt K, Fourmentin M, Choel M. Atmospheric reactivity of hydroxyl radicals with guaiacol (2- methoxyphenyl), a biomass burning emitted compound: secondary organic aerosol formation and gas-phase oxidation products. Atmos Environ 2014; 86: 155-163
• 44 Gülçin I, Beydemir Ş. Phenolic compounds as antioxidants: carbonic anhydrase isoenzymes inhibitors. Mini Rev Med Chem 2013; 13 (03) 408-430
• 45 Varfolomeev MA, Abaidullina DI, Solomonov BN, Verevkin SP, Emel'yanenko VN. Pairwise substitution effects, inter- and intramolecular hydrogen bonds in methoxyphenols and dimethoxybenzenes. Thermochemistry, calorimetry, and first-principles calculations. J Phys Chem B 2010; 114 (49) 16503-16516
• 46 Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Advances 2015; 5: 27986-28006
• 47 Haan JB, Jandeleit-Dahm KA, Allen TJ. Role of Oxidative Stress and Targeted Antioxidant Therapies in Experimental Models of Diabetic Complications. Springer Science. 2011 doi10.1007/978-1-60761-956-7
• 48 Huang MH, Chang LW, Sung WC, Vong WJ, Wang BS. Protective effects of three smoke flavouring phenols on oxidative damage and nitric oxide production. Food Chem 2011; 126 (04) 1655-1661
• 49 Ashor AW, Siervo M, Lara J, Oggioni C, Afshar S, Mathers JC. Effect of vitamin C and vitamin E supplementation on endothelial function: a systematic review and meta-analysis of randomised controlled trials. Br J Nutr 2015; 113 (08) 1182-1194
• 50 Tracy LE, Minasian RA, Caterson EJ. Extracellular matrix and dermal fibroblast function in the healing wound. Adv Wound Care (New Rochelle) 2016; 5 (03) 119-136
• 51 Desta T, Li J, Chino T, Graves DT. Altered fibroblast proliferation and apoptosis in diabetic gingival wounds. J Dent Res 2010; 89 (06) 609-614
• 52 Sun K, Wang W, Wang C. et al. AGEs trigger autophagy in diabetic skin tissues and fibroblasts. Biochem Biophys Res Commun 2016; 471 (03) 355-360
• 53 Garcia-Bailo B, El-Sohemy A, Haddad PS. et al. Vitamins D, C, and E in the prevention of type 2 diabetes mellitus: modulation of inflammation and oxidative stress. Biologics 2011; 5: 7-19