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CC BY-NC-ND 4.0 · Eur J Dent 2019; 13(02): 206-212
DOI: 10.1055/s-0039-1694305
Original Article
Dental Investigation Society

Osteogenic Differentiation and Biocompatibility of Bovine Teeth Scaffold with Rat Adipose-derived Mesenchymal Stem Cells

Desi Sandra Sari
1   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
2   Department of Periodontics, Faculty of Dentistry, Universitas Jember, Jember, Indonesia
,
Ernie Maduratna
3   Department of Periodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ferdiansyah,
Fourier Dzar Eljabbar Latief
6   Micro-CT Laboratory, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung, Indonesia
,
Satuman,
Alexander Patera Nugraha
1   Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
5   Stem Cells Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
8   Department of Orthodontics, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Ketut Sudiana
9   Department of Microscope Electron, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
10   Department of Pathology Anatomy, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fedik Abdul Rantam
5   Stem Cells Research and Development Center, Universitas Airlangga, Surabaya, Indonesia
11   Laboratory of Virology, Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia
› Author Affiliations Funding The research was funded by doctoral dissertation research, Direktorat Jenderal Penguatan Riset dan Pengembangan, The Ministry of Research, Technology and Higher Education of The Republic of Indonesia (Kemenristekdikti RI letter of appointment agreement number 058.SP2H/LT/DRPM/2018.
Further Information

Publication History

Publication Date:
16 September 2019 (online)

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Abstract

Objective Adipose-derived mesenchymal stem cells (ADMSCs) have great potential for regenerative medicine. These have been combined with biomaterials such as Bovine teeth that are preferred as a periodontal regeneration material. The main purpose of this study is to evaluate and analyze a biocompatibility test and osteogenic differentiation of bovine teeth scaffold seeded with ADMSCs in vitro.

Materials and Methods A true experimental study with post-test only group design was conducted. Random sampling and Lameshow's formula were used to determine the sample. The scaffold, obtained from bovine teeth as the bone graft material, was analyzed using 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and its attachment was evaluated by scanning electron microcopy (SEM) and micro-computed tomography with ADMSCs. ADMDSCs attachment present in the bovine teeth scaffold was assessed using SEM at 1-hour, 12-hour, and 24-hour intervals.

Statistical Analysis Analysis of variance was used to analyze the MTT assay results (p < 0.05) based on normality and homogeneity test (p > 0.05).

Results The highest viability of cells (97.08%) was found at a concentration of 10% by means of an MTT test (p < 0.05). The results of three-dimensional bovine teeth scaffold showed the average particle size to be 500 µm. ADMSCs cell attachment to the scaffold bovine teeth showed a significant increase in the number of cells attached after 24 hours compared with those at 1 and 12 hours. Alizarin red staining showed an increase in ADMSC osteogenic differentiation after it was combined with bovine teeth scaffold.

Conclusion The scaffold from bovine teeth is biocompatible and accelerates osteogenic differentiation of ADMSC.

Keywords

adipose-derived mesenchymal stem cells - bovine teeth - scaffold - tissue engineering
 

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