A New Technique for Research on Wound Healing through Extraction of Mandibular Lower Incisors in Wistar Rats

Abstract

Objective The aim of this study was to explain a new technique for research on wound healing through extraction of mandibular lower incisors in Wistar rats.

Materials and Methods Fifty Wistar rats were used from studies using the experimental animal to investigate the effects of wound healing drugs on alveolar bone. The mandibular incisors of the Wistar rats were extracted using a special modified bein and special pulling pliers under general anesthesia. The tooth socket after extraction was closed using a 5.0 suture nylon needle.

Results The results of this technique used in this extraction showed 2% incisor tooth fracture and 3% bleeding, and that all fractured teeth could be removed properly, and bleeding could be stopped after suturing.

Conclusion The new techniques we use can be useful for research on mucosal and alveolar bone healing, specifically finding new types of drugs to accelerate wound healing after alveolar bone post extraction.

Keywords

tooth extraction - mandibular incisors - alveolar bone healing

• References

• 1 Balqis U, Darmawi. Iskandar CD, Salim MN. Angiogenesis activity of. Jatropha curcas. L. latex in cream formulation on wound healing in mice. Vet World 2018; 11 (07) 939-943
• 2 Yuniarti WM, Lukiswanto BS. Effects of herbal ointment containing the leaf extracts of Madeira vine (Anredera cordifolia (Ten.) Steenis) for burn wound healing process on albino rats. Vet World 2017; 10 (07) 808-813
• 3 Valizadeh R, Hemmati AS, Houshmand G, Bayat S, Bahadoram M. Wound healing potential of Althaea officinalis flower mucilage in rabbit full thickness wounds. Asian Pac J Trop Biomed 2015; 5 (11) 937-943
• 4 Bohling MW, Henderson RA. Differences in cutaneous wound healing between dogs and cats. Vet Clin North Am Small Anim Pract 2006; 36 (04) 687-692
• 5 Graziani F, Laurell L, Tonetti M, Gottlow J, Berglundh T. Periodontal wound healing following GTR therapy of dehiscence-type defects in the monkey: short-, medium- and long-term healing. J Clin Periodontol 2005; 32 (08) 905-914
• 6 Zecchin KG, Jorge RDS, Jacks JorgeJ. A new method for extraction of mandibular first molars in rats. Braz J Oral Sci 2007; 6 (21) 1344-1348
• 7 Khoswanto C, Soehardjo I. The effect of Binahong Gel (Anredera cordifolia (Ten.) Steenis) in accelerating the escalation expression of HIF-1α and FGF-2. Journal of International Dental and Medical Research 2018; 11 (01) 303-307
• 8 Günay A, Arpağ OF, Atilgan S, Yaman F, Atalay Y, Acikan I. Effects of caffeic acid phenethyl ester on palatal mucosal defects and tooth extraction sockets. Drug Des Devel Ther 2014; 8: 2069-2074
• 9 Meerlo P, Overkamp GJ, Benning MA, Koolhaas JM, Van den Hoofdakker RH. Long-term changes in open field behaviour following a single social defeat in rats can be reversed by sleep deprivation. Physiol Behav 1996; 60 (01) 115-119
• 10 Hong YP, Lee HC, Kim HT. Treadmill exercise after social isolation increases the levels of NGF, BDNF, and synapsin I to induce survival of neurons in the hippocampus, and improves depression-like behavior. J Exerc Nutrition Biochem 2015; 19 (01) 11-18
• 11 Prasanth T, Saraswathi T. Histopathological and radiographic evaluation of rat molar teeth after traumatic injury-a pilot study. J Oral Maxillofac Pathol 2012; 16 (03) 313-317
• 12 Rahman MNA, Sukmasari S, Doolaanea AA, Qader OAJA. Potential oral wound healing of topical application of dental gel prepared from Baccaurea angulate fruit in diabetic rats. J Pharm Sci Res 2018; 10 (01) 167-174
• 13 Matsuzawa H, Toriya N, Nakao Y. et al. Cementocyte cell death occurs in rat cellular cementum during orthodontic tooth movement. Angle Orthod 2017; 87 (03) 416-422
• 14 Ho SP, Yu B, Yun W, Marshall GW, Ryder MI, Marshall SJ. Structure, chemical composition and mechanical properties of human and rat cementum and its interface with root dentin. Acta Biomater 2009; 5 (02) 707-718