Screening of Antiseizure Activity of Aryl Acetic Acid Compounds in Wistar Albino Rats by using PTZ and MES Tests

Objective: To screen the antiseizure activity of aryl acetic acid compound in Wistar albino rats using PTZ and MES tests.

Methods: After obtaining the approval from Institutional Review Board (IRB), the healthy adult Wistar albino rats of either sex weighing between 180 and 250 g were procured from the central animal house of the Institution. The CPCSEA guidelines were followed for conducting the experiments on animals. The animals were housed in the research laboratory for 1 week before initiation of study. The animals were then randomly selected and divided into control, standard, and test groups (n = 6). Group I: control for PTZ; group II: standard for PTZ (sodium valproate, i.p.); group III: aryl acetic acid compound for PTZ (i.p.); group IV: control for MES; group V: standard for MES (diphenylhydantoin, i.p.); and group VI: aryl acetic acid compound for MES (i.p.). One-way ANOVA with Bonferroni's post hoc test was used for statistical significance.

Results: The standard drug sodium valproate in group II and test drug aryl acetic acid compound in group III showed significant reduction in onset, duration, and number of seizures when compared with the group I (control). Similarly, the standard drug diphenylhydantoin in group V and test drug aryl acetic acid compound in group VI showed significant reduction in scores of seizures and THLE when compared with the group IV (control).

Conclusion: The experimental drug aryl acetic acid compound had significant antiseizure activity in both PTZ and MES tests in Wistar albino rats.