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CC BY-NC-ND 4.0 · International Journal of Epilepsy 2018; 05(02): S13-S14
DOI: 10.1055/s-0039-1694892
Abstracts of 20th Joint Annual Conference of Indian Epilepsy Society and Indian Epilepsy Association (ECON 2019)
Indian Epilepsy Society

Effect of T-Type of Calcium Channel Blockers on Behavioral, Biochemical, Immunohistochemical, Oxidative, and Histopathological Parameters in Chemically Induced Seizure Tests in Wistar Albino Rats

Saniya K.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Patil B. G.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Madhavrao C.‡
2   Department of Pharmacology, All India Institute of Medical Sciences, Delhi, India
,
Prakash K. G.‡
1   Department of Anatomy, All India Institute of Medical Sciences, Delhi, India
,
Mythili Bai K.‡
3   Department of Physiology, All India Institute of Medical Sciences, Delhi, India
› Author Affiliations
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Publication Date:
31 July 2019 (online)

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Objective: To study the effect of t-type of calcium channel blockers on behavioral, biochemical, immunohistochemical, oxidative, and histopathological parameters in chemically induced seizure tests in Wistar albino rats.

Methods: The study proposal was approved by the Institutional Review Board (IRB) and all study procedures were performed in accordance with the CPCSEA guidelines. The study was done on the healthy, adult Wistar albino rats of either sex as per the standard protocol described in the literature. This study consisted of four groups, each with six animals. The study groups were group I (vehicle control), group II (negative control), group III (positive control), and group IV (experimental drug [t-type of calcium channel blocker: Flunarizine]). Statistical software, GraphPad Instat 3.0 version was used to confirm the statistically significant difference among groups at p-value of less than 0.05.

Results: It was clearly evident from the obtained data that, experimental test drug and standard drug, both had significant increase in onset (p < 0.01), decline in duration (p < 0.05), score (p < 0.01), and number of seizures in 1 hour time (p < 0.05) in pentylenetetrazole-induced seizure model tests. The various parameters including biochemical, immunohistochemical, histopathological, and oxidative stress (SOD, catalase, GPx, and lipid peroxidation) were found in favor of decreasing the neuronal excitability and protecting the neurons with the experimental test drug in group IV with statistical significance (p < 0.01) when compared with the control group. The above findings in group IV were consistent and comparable to the standard drug in group III (p > 0.05).

Conclusion: The t-type of calcium channel blocker had significant ameliorative effect on behavioral, biochemical, immunohistochemical, oxidative, and histopathological parameters in chemically induced seizure tests in Wistar albino rats.