ABSTRACT
Objectives: The aim of this study was to evaluate the effects of five self-etch dental
composite resin cements on the cell viability of bovine dental papilla-derived cells.
Methods: The cytotoxicity of composite resin cements (Rely X Unicem Clicker, 3M ESPE;
MaxCem; KERR, PanaviaF 2.0; Kuraray, BisCem; Bisco and Bistite II DC; Tokuyama) was
analyzed in a dentin barrier test device using three-dimensional (3D) pulp cell cultures.
A commercially available cell culture perfusion chamber was separated into two compartments
by 500 im dentin disc. The three dimensional cultures placed on a dentin disk held
in place by a special biocompatible stainless-steel holder. Test materials were introduced
into the upper compartment in direct contact with the cavity side of the dentin disks
according to the manufacturer’s instructions. Subsequently, the pulpal part of the
perfusion chamber containing the cell cultures was perfused with medium (2 ml/h).
After an exposure period of 24 h, the cell survival was determined by the MTT assay.
Statistical analyses were performed using the Mann–Whitney U-test.
Results: In dentin barrier test, cell survival was similar with Maxcem and negative
control group (P>.05), and all other tested materials were cytotoxic for the three
dimensional cell cultures (P>.05).
Conclusions: The significance of composite resin cements is being more important in
dentistry. The cytotoxic potencies demonstrated by these materials might be of clinical
relevance. Some composite resin cements include biologically active ingredients and
may modify pulp cell metabolism when the materials are used in deep cavities or directly
contact pulp tissue. (Eur J Dent 2009;3:120-126)
Key words:
Resin cement - Cytotoxicity - Dentin barrier test - Three dimensional cultures
