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CC BY-NC-ND 4.0 · Eur J Dent 2007; 01(02): 086-090
DOI: 10.1055/s-0039-1698319
Original Article
Dental Investigation Society

The Release of Elements from Dental Casting Alloy into Cell-Culture Medium and Artificial Saliva

Gülşen Can
a   Department of Prosthodontics, Faculty of Dentistry, University of Ankara
,
Gül Akpınar
b   SSYB Osmanlı Dental Treatment and Prosthetic Department
,
Ahmet Aydın
c   Department of Pharmaceutical Toxicology Gülhane Military Medical Academy
› Author Affiliations
Further Information

Publication History

Publication Date:
27 September 2019 (online)

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ABSTRACT

Objectives: The biocompatibility of dental casting alloys is a critical issue because these alloys are in long-term intimate contact with oral tissues. Since the biocompatibility of alloys is not completely known; the release of elements from the alloys has been studied. The aim of this study was to compare the elemental release from dental casting alloy during exposure to artificial saliva and cell-culture medium.

Materials and Methods: Twenty specimens made from Ni-Cr alloy were provided in the form of 5 mm diameter discs, 2 mm in thickness with a 7 mm stem attached to one face to facilitate handling. Ten of twenty samples were polished separately using a conventional technique. The remaining ten samples were left sandblasted with 50 μm Al203. Ten samples (5 polished, 5 sandblasted) were separately placed into cell-culture wells with Dulbecco’s Modified Eagle’s Medium. The other ten samples were placed separately into cell-culture wells with artificial saliva. The samples were subjected in contact with these medium for 30 days. These medium were collected every 7 days. The cell-culture medium and artificial saliva without alloy samples were subjected to elemental analyses as a control. At the end of the exposure time, Atomic Absorption Spectrometry (AAS) was used to determine the release of elements from the alloys into all collected medium. Statistical analyses were assessed with two-way ANOVA

Results: In general, the elemental release occurred with in all medium. The elemental releases of sandblasted alloys were higher than polished alloys. Artificial saliva was found to cause more release from the samples. In both media, Ni released from polished and sandblasted alloys were higher than Cr and Mo.

The results suggest that the release of elements from the alloys might have correlated with the environments and the surface of dental alloy. (Eur J Dent 2007;2:86-90)

Key words

Dental alloy - Ni-Cr alloy - Elemental release - Biocompatibility - Cell-culture medium - Artificial saliva
 

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