ETHANOLIC EXTRACT OF NARDOSTACHYS JATAMANSI POTENTIATES HAEMATOPOIETIC SYSTEM IN ALBINO WISTAR RATS

Damodara Gowda K. M.; Lathika Shetty; Krishna A. P.; Suchetha Kumari N.; Ganesh Sanjeev; Naveen P.; Sharada V.

doi:10.1055/s-0040-17036329

Journal of Health and Allied Sciences NU, Table of Contents

CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2013; 03(01): 25-29
DOI: 10.1055/s-0040-17036329

Original Article

ETHANOLIC EXTRACT OF NARDOSTACHYS JATAMANSI POTENTIATES HAEMATOPOIETIC SYSTEM IN ALBINO WISTAR RATS

Authors

Damodara Gowda K. M.

¹Department of Physiology, K. S. Hegde Medical Academy, Nitte University, Mangalore - 575 018. Karnataka, India
Lathika Shetty

²Department of Radiology, K. S. Hegde Medical Academy, Nitte University, Mangalore - 575 018. Karnataka, India
Krishna A. P.

¹Department of Physiology, K. S. Hegde Medical Academy, Nitte University, Mangalore - 575 018. Karnataka, India
Suchetha Kumari N.

³Department of Biochemistry, K. S. Hegde Medical Academy, Nitte University, Mangalore - 575 018. Karnataka, India
Ganesh Sanjeev

⁴Department of Physics, Microtron centre, Mangalore University, Karnataka, India - 574 199. Nitte University, Mangalore, Karnataka, India - 575 018.
Naveen P.

⁵Central Research Laboratory Nitte University, Mangalore, Karnataka, India - 575 018.
Sharada V.

⁶Department of Community Medicine, Microtron centre, Mangalore University, Karnataka, India - 574199

Abstract

PDF Download

Abstract

Background and Objective: The consumption of a variety of local herbs and vegetables by man contributes significantly to the improvement of human health in terms of prevention, and/or cure of diseases because plants have long served as a useful and natural source of therapeutic agents. The present study aims at investigating the hematopoietic effect of Nardostachys jatamansi extract (NJE) in albino wistar rats.

Materials and Methods: Twenty four male albino wistar rats were used and divided into 4 groups of 6 animals each. The Group I served as a normal control. The animals of Group II, III and IV were administered orally with aqueous suspension of NJE at the dosage of 100 mg/kg, 200 mg/kg and 400 mg/kg body weight for 15 consecutive days respectively. Then blood was Blood was collected and used for the estimation of Peripheral blood counts (RBC, WBC), haemoglobin, thrombocyte count and Hematocrit was determined at day 0.25 (6 hrs), 0.5 (12 hrs), 1, 2, 5, 10, and 15 days of treatment using automated haematology analyzer. Body weight was also recorded regularly. Statistical Analysis was done by one way ANOVA followed by Bonferroni post hoc test for multiple comparisons using SPSS-16. P value less than 0.05 was considered the level of significance.

Results: All the parameters have shown a significant increase (p = 0.000) in experimental animals except body weight which was increased insignificantly.

Conclusion: Nardostachys jatamansi extract can be attributed to stimulating or protecting hematopoiesis.

Keywords

Nardostachys jatamansi - Peripheral Blood Counts - Haemoglobin - Thrombocyte Count - Hematocrit

PDF (126 kb)

References

References
1 Anand N. Comprehensive medical chemistry.1990; Pergamon press. Oxford. Vol.1, p.113.
2 Dada A and Ikuerowo M. Effects of ethanolic extracts of Garcinia kola seeds on growth and haematology of catfish (Clarias gariepinus)broodstock. African Journal of Agricultural Research.2009; Vol. 4(4): pp. 344-347,.
3 Nishteswar K. Text book of Dravyaguna. 2007; Chaukhamba publishing house, Darya Ganj, Delhi, p. 250-251.
4 Sharma PV. Dravyaguna Vignana. 2001; Chankhamba publishing house, Darya Ganj, Delhi, Vol. 2: p. 31-34.
5 Sukhdev. Ethnotherapeutics and modern drug development: Potential of Ayurveda. Current Science. 1997; 73: 920.
6 Ajayi OI, Ajayi TC, Omokaro EU, Halim NK. Erythropoeitic value of pumpkin, Telfeira occidentalis leaf extract in rabbits- a preliminary study. Nigerian Journal of Physiological Sciences. 2000; 16(1-2):1-3.
7 Ogwumike OO. Hemopoietic effect of aqueous extract of the leaf sheath of Sorghum bicolor in albino rats. African Journal of Biomedical Research 2002; 5:6971.
8 Owoyele BY, Olayele SB, Elegba RA. Anti-inflammatory and Analgesic Activities of leaf Extract of Landolphia owariensis. African J. Biomedical Res. 2002; 4(3):131-133.
9 Agbor GA, Oben JE, Ngogang JY. Hematinic activity of Hibiscus cannabinus. Afr J Biotechnology. 2005;4(8):833-837.
10 Dina OA, Adedapo AA, Oyinloye OP, Saba AB. Effect of Telfairia occidentalis extract on experimentally induced anemia in domestic rabbits. Afr J Biomed Res 2006; 3:181-183.
11 Ita SO, Etim OE, Ben EE, Ekpo OF. Hematopoietic properties of ethanolic leaf extract of Ageratum conyzoides (Goat weed) in albino rats. Nigerian Journal of Physiological Sciences 2007; 22(1-2):8387.
12 Akah PE, Okolo CE, Ezike AE. The haematinic activity of the methanol leaf extract of Brillantasia nitens Lindau (Acanthaceae) in rats. African Journal of Biotechnology 2009; 8(10):2389-2393.
13 Dacie JV, Lewis SM. Practical Hematology, 8th ed., Longman Group Ltd, Hong Kong. 1994; p.49-82.
14 Okochi YI, Okpuzor J, Alli LA. Comparison of an African herbal formula with commercially available haematinics. Afr J Biotechnol 2003; 2(8):237-240.
15 Diallo A, Gbeassor M, Vovor A, Eklu-Gadegbeku K, Aklikokou K, Agbonon A, Abena AA, et al. Effect of Tectona grandis on phenylhydrazine-induced anaemia in rats. Fitoterapia 2008; 79(5):332-336.
16 Okonkwo JE, Iyadi KC, Effiong CO. Effect of chronic administration of haematological parameters of rats. Nig J Physiol Sc 2004; 19(1-2):10-13.
17 Friday E. Uboh, Iniobong E. Okon, Moses B. Ekong Effect of Aqueous Extract of Psidium guajava Leaves on Liver Enzymes, Histological Integrity and Hematological Indices in Rats. Gastroenterology Research. 2010; Vol 3, No.1: p. 32-38.
18 Chris Wambi, Jenine Sanzari Steven Wani, Manunya Nuth, James Davis. Dietary Anti-oxidant Protect Hematopoietic Cells and Improve Animal Survival after Total-Body Irradiation. Radial Res.2008; 169 (4):384-396.
19 Jaja, SI, Ikotun AR, Gbenebites S, Teniye E. Blood pressure hematologic and erythrocyte fragility changes in children suffering from sickle cell anaemia following ascorbic acid supplementation. J. Trop. Paed., 2002; 48:4203-4206.
20 North TE, Goessling W, Walkley CR, Lengerke C, Kopani KR, Lord AM, et al. Prostaglandin E regulates vertebrate haematopoietic stem cell 2 homeostasis. Nature. 2007; 447:1007-1011.
21 Ekpenyong CE, Akpan UP, Ben EE, Nwama EO, Ibu JO. Hematological effect of chronic administration of ethanolic extract of Garcinia conruana seed on rat. J Nat Prod.2011; 4:173-176.