Anti-Inflammatory Effect of Musa acuminata Stem

Abstract

Objective This study was designed to assess the anti-inflammatory effect of Musa acuminata through the expression of tumor necrosis factor-α (TNF-α) and nuclear factor kappa β (NF-κB) after 3 days of application of Musa acuminata stem extract (MASE) gel on oral mucosal wound.

Materials and Methods An experimental study with post-test only control group design was conducted. Twenty male Rattus norvegicus (Wistar) were injured on their left buccal mucosa and treated three times a day with MASE gel of varying concentrations: 0% (as control), MASE 25%, MASE 37.5%, and MASE 50%. On day 3, a biopsy was performed on each mucosal wound for later immunohistochemical analysis for the expressions of TNF-α and NF-κB.

Results The highest expression of TNF-α was observed in the control group (13.20 ± 1.79), while the lowest was in the treatment group using 50% MASE (6.40 ± 1.14). Meanwhile the comparison between treatment groups did not highlight any significant difference (p > 0.05). The highest expression of NF-κB was observed in the control group (13.20 ± 1.30), whereas the lowest was in the treatment group using MASE 50% (6.40 ± 1.14). NF-κB was significantly lower in the treatment group using MASE 50% when compared with other treatment groups (p < 0.05).

Conclusion Application of MASE on mucosal wound reduces the expression of TNF-α and NF-κB at all concentrations. The anti-inflammatory effect of MASE 50% was the strongest one.

Keywords

Musa acuminata - anti-inflammation - TNF-α - NF-κB

Publication History

Article published online:
12 May 2020

• References

• 1 Maver T, Maver U, Stana Kleinschek K, Smrke DM, Kreft S. A review of herbal medicines in wound healing. Int J Dermatol 2015; 54 (07) 740-751
• 2 Apriasari ML, Iskandar SE. Bioactive compound and antioxidant activity of methanol extract Mauli bananas (Musa sp) stem. Int J Biosci Biochem Bioinform 2014; 4 (02) 110-115
• 3 Fitriati Noor W, Aprianti N, Rizky Saputra S, Laillyza Apriasari M, Suhartono E. Oxidative stress on buccal mucosa wound in rats and rule of topical application of ethanolic extracts of Mauli banana (Musa acuminata) stem. J Trop Life Sci 2015; 5 (02) 84-87
• 4 Apriasari ML, Endariantari A, Oktaviyanti IK. The effect of 25% Mauli banana stem extract gel to increase the epithel thickness of wound healing process in oral mucosa. Dent J 2015; 48 (03) 151-154
• 5 Apriasari ML, Dachlan YP, Ernawati DS. Effect of musa acuminata stem by immunohistochemistry test in ulcer. Asian J Biochem 2016; 11 (03) 135-141
• 6 Aggarwal BB, Gupta SC, Sung B. Curcumin: an orally bioavailable blocker of TNF and other pro-inflammatory biomarkers. Br J Pharmacol 2013; 169 (08) 1672-1692
• 7 Surboyo MDC, Arundina I, Rahayu RP, Mansur D, Bramantoro T. Potential of distilled liquid smoke derived from coconut (Cocos nucifera L) shell for traumatic ulcer healing in diabetic rats. Eur J Dent 2019; 13 (02) 271-279
• 8 Surboyo MDC, Ernawati DS, Arundina I, Rahayu RP. Oral ulcer healing after treatment with distilled liquid smoke of coconut shell on diabetic rats. J Krishna Inst Med Sci Univ 2019; 8 (02) 70-79
• 9 Liu T, Zhang L, Joo D, Sun SC. NF-κB signaling in inflammation. Signal Transduct Target Ther 2017; 2
• 10 Gonzalez ACDO, Costa TF, Andrade ZA, Medrado ARP. Wound healing - a literature review. An Bras Dermatol 2016; 91 (05) 614-620
• 11 Janssen WJ, Henson PM. Cellular regulation of the inflammatory response. Toxicol Pathol 2012; 40 (02) 166-173
• 12 Qing C, The molecular biology in wound healing & non-healing wound [Internet]. Vol. 20, Chinese Journal of Traumatology - English Edition. Daping Hospital and the Research Institute of Surgery of the Third Military Medical University; 2017 p. 189–93. Available at: http://dx.doi.org/10.1016/j. cjtee.2017.06.001
• 13 Brancato SK, Albina JE. Wound macrophages as key regulators of repair: origin, phenotype, and function. Am J Pathol 2011; 178 (01) 19-25 [Internet]
• 14 Zhang JM, An J. Cytokines, inflammation, and pain. Int Anesthesiol Clin 2007; 45 (02) 27-37
• 15 Lawrence T. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 2009; 1 (06) a001651
• 16 Ritsu M, Kawakami K, Kanno E. et al. Critical role of tumor necrosis factor-αin the early process of wound healing in skin. J Dermatology Dermatologic Surg [Internet] 2017; 21 (01) 14-19
• 17 Han YP, Tuan TL, Wu H, Hughes M, Garner WL. TNF-α stimulates activation of pro-MMP2 in human skin through NF-(κ)B mediated induction of MT1-MMP. J Cell Sci 2001; 114 (Pt 1) 131-139
• 18 Pires BR, Silva RCMC, Ferreira GM, Abdelhay E. NF-kappaB: two sides of the same coin. Genes (Basel) 2018; 9 (01) E24
• 19 Kim JH, Gupta SC, Park B, Yadav VR, Aggarwal BB. Turmeric (Curcuma longa) inhibits inflammatory nuclear factor (NF)-κB and NF-κB-regulated gene products and induces death receptors leading to suppressed proliferation, induced chemosensitization, and suppressed osteoclastogenesis. Mol Nutr Food Res 2012; 56 (03) 454-465