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CC BY-NC-ND 4.0 · Rev Bras Ortop (Sao Paulo) 2021; 56(03): 326-332
DOI: 10.1055/s-0040-1712139
Artigos Originais
Joelho

Avaliação do biomarcador CTX-II em pacientes com ruptura do ligamento cruzado anterior: Estudo piloto[*]

Article in several languages: português | English
Alexandre Pedro Nicolini 
1   Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Nacime Salomão Barbachan Mansur
1   Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Juliana Luporini Dreyfuss
2   Divisão de Biologia Molecular, Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Benno Ejnisman
1   Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Moises Cohen
1   Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
,
Diego Costa Astur
1   Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil
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Resumo

Objetivo Quantificar a concentração urinária do biomarcador telopeptídeo C de ligação cruzada de colágeno de tipo II (C-terminal cross-linked telopeptide of type-II collagen, CTX-II) em casos de lesão isolada do ligamento cruzado anterior (LCA), e comparar as concentrações observadas nessa população com um grupo controle composto por pacientes sem alterações metabólicas no joelho que possam levar à degeneração da cartilagem.

Métodos Este é um estudo piloto transversal com dois grupos: pacientes com ruptura do LCA e grupo controle (cada grupo era composto por 10 indivíduos do sexo masculino, com 18 a 35 anos de idade, e índice de massa corporal inferior a 30 kg/m2). Nos dois grupos, as concentrações urinárias de um biomarcador relacionado à degradação do colágeno de tipo II (CTX-II) foram medidas. No grupo com ruptura do LCA, a relação entre o tempo pós-lesão e a quantidade do biomarcador também foi analisada.

Resultados Houve diferenças significativas nas concentrações urinárias de CTX-II entre o grupo LCA e o grupo controle (p = 0,009). Não foi observada relação significativa entre o tempo de lesão e a quantidade do biomarcador.

Conclusões Pacientes com lesão do LCA apresentaram maiores concentrações urinárias do biomarcador CTX-II do que aqueles sem lesão do LCA (p = 0,009). No entanto, não houve correlação entre a concentração desse biomarcador e o tempo decorrido após a lesão (p> 0,05).

Palavras-chave

osteoartrite - biomarcadores - lesões do ligamento cruzado anterior

* Trabalho desenvolvido no Departamento de Ortopedia e Traumatologia, Centro de Traumatologia do Esporte, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.


Suporte Financeiro

Não houve suporte financeiro de fontes públicas, comerciais, ou sem fins lucrativos.




Publication History

Received: 12 November 2019

Accepted: 02 March 2020

Article published online:
22 July 2020

© 2020. Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • Referências

  • 1 Sanders TL, Maradit Kremers H, Bryan AJ. et al. Incidence of Anterior Cruciate Ligament Tears and Reconstruction: A 21-Year Population-Based Study. Am J Sports Med 2016; 44 (06) 1502-1507
    CrossrefPubMedGoogle Scholar
  • 2 Poole AR, Kobayashi M, Yasuda T. et al. Type II collagen degradation and its regulation in articular cartilage in osteoarthritis. Ann Rheum Dis 2002; 61 (Suppl. 02) ii78-ii81
    CrossrefPubMedGoogle Scholar
  • 3 Lohmander LS, Atley LM, Pietka TA, Eyre DR. The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis. Arthritis Rheum 2003; 48 (11) 3130-3139
    CrossrefPubMedGoogle Scholar
  • 4 Bigoni M, Sacerdote P, Turati M. et al. Acute and late changes in intraarticular cytokine levels following anterior cruciate ligament injury. J Orthop Res 2013; 31 (02) 315-321
    CrossrefPubMedGoogle Scholar
  • 5 Palmieri-Smith RM, Wojtys EM, Potter HG. Early Cartilage Changes After Anterior Cruciate Ligament Injury: Evaluation With Imaging and Serum Biomarkers-A Pilot Study. Arthroscopy 2016; 32 (07) 1309-1318
    CrossrefPubMedGoogle Scholar
  • 6 Wang X, Bennell KL, Wang Y. et al. Tibiofemoral joint structural change from 2.5 to 4.5 years following ACL reconstruction with and without combined meniscal pathology. BMC Musculoskelet Disord 2019; 20 (01) 312
    CrossrefPubMedGoogle Scholar
  • 7 Cohen M, Amaro JT, Ejnisman B. et al. Anterior cruciate ligament reconstruction after 10 to 15 years: association between meniscectomy and osteoarthrosis. Arthroscopy 2007; 23 (06) 629-634
    CrossrefPubMedGoogle Scholar
  • 8 Chmielewski TL, Trumble TN, Joseph AM. et al. Urinary CTX-II concentrations are elevated and associated with knee pain and function in subjects with ACL reconstruction. Osteoarthritis Cartilage 2012; 20 (11) 1294-1301
    CrossrefPubMedGoogle Scholar
  • 9 Oiestad BE, Holm I, Aune AK. et al. Knee function and prevalence of knee osteoarthritis after anterior cruciate ligament reconstruction: a prospective study with 10 to 15 years of follow-up. Am J Sports Med 2010; 38 (11) 2201-2210
    CrossrefPubMedGoogle Scholar
  • 10 Bauer DC, Hunter DJ, Abramson SB. et al. Osteoarthritis Biomarkers Network. Classification of osteoarthritis biomarkers: a proposed approach. Osteoarthritis Cartilage 2006; 14 (08) 723-727
    CrossrefPubMedGoogle Scholar
  • 11 Fosang AJ, Stanton H, Little CB, Atley LM. Neoepitopes as biomarkers of cartilage catabolism. Inflamm Res 2003; 52 (07) 277-282
    CrossrefPubMedGoogle Scholar
  • 12 Øiestad BE, Engebretsen L, Storheim K, Risberg MA. Knee osteoarthritis after anterior cruciate ligament injury: a systematic review. Am J Sports Med 2009; 37 (07) 1434-1443
    CrossrefPubMedGoogle Scholar
  • 13 Mobasheri A, Henrotin Y. Biomarkers of (osteo)arthritis. Biomarkers 2015; 20 (08) 513-518
    CrossrefPubMedGoogle Scholar
  • 14 Cameron M, Buchgraber A, Passler H. et al. The natural history of the anterior cruciate ligament-deficient knee. Changes in synovial fluid cytokine and keratan sulfate concentrations. Am J Sports Med 1997; 25 (06) 751-754
    CrossrefPubMedGoogle Scholar
  • 15 Harkey MS, Luc BA, Golightly YM. et al. Osteoarthritis-related biomarkers following anterior cruciate ligament injury and reconstruction: a systematic review. Osteoarthritis Cartilage 2015; 23 (01) 1-12
    CrossrefPubMedGoogle Scholar
  • 16 Ajuied A, Wong F, Smith C. et al. Anterior cruciate ligament injury and radiologic progression of knee osteoarthritis: a systematic review and meta-analysis. Am J Sports Med 2014; 42 (09) 2242-2252
    CrossrefPubMedGoogle Scholar
  • 17 Higuchi H, Shirakura K, Kimura M. et al. Changes in biochemical parameters after anterior cruciate ligament injury. Int Orthop 2006; 30 (01) 43-47
    CrossrefPubMedGoogle Scholar
  • 18 Lohmander LS, Englund PM, Dahl LL, Roos EM. The long-term consequence of anterior cruciate ligament and meniscus injuries: osteoarthritis. Am J Sports Med 2007; 35 (10) 1756-1769
    CrossrefPubMedGoogle Scholar
  • 19 Mouritzen U, Christgau S, Lehmann HJ, Tankó LB, Christiansen C. Cartilage turnover assessed with a newly developed assay measuring collagen type II degradation products: influence of age, sex, menopause, hormone replacement therapy, and body mass index. Ann Rheum Dis 2003; 62 (04) 332-336
    CrossrefPubMedGoogle Scholar
  • 20 Jorge PB, Sprey JW, Runco GM, Lima MV, Severino NR, Santili C. Diferença na degeneração articular de acordo com o tipo de esporte. Rev Bras Ortop 2019; 54 (05) 509-515
    Article in Thieme ConnectPubMedGoogle Scholar
  • 21 Severino RM, Jorge PB, Martinelli MO, de Lima MV, Severino NR, Duarte Junior A. Análise dos níveis séricos do biomarcador CTX-II em atletas profissionais como fator preditivo de degradação articular. Rev Bras Ortop 2015; 50 (03) 331-335
    CrossrefPubMedGoogle Scholar
  • 22 Wu W, Billinghurst RC, Pidoux I. et al. Sites of collagenase cleavage and denaturation of type II collagen in aging and osteoarthritic articular cartilage and their relationship to the distribution of matrix metalloproteinase 1 and matrix metalloproteinase 13. Arthritis Rheum 2002; 46 (08) 2087-2094
    CrossrefPubMedGoogle Scholar
  • 23 Nelson F, Billinghurst RC, Pidoux I. et al. Early post-traumatic osteoarthritis-like changes in human articular cartilage following rupture of the anterior cruciate ligament. Osteoarthritis Cartilage 2006; 14 (02) 114-119
    CrossrefPubMedGoogle Scholar
  • 24 Irie K, Uchiyama E, Iwaso H. Intraarticular inflammatory cytokines in acute anterior cruciate ligament injured knee. Knee 2003; 10 (01) 93-96
    CrossrefPubMedGoogle Scholar
  • 25 Davis HC, Spang JT, Loeser RF. et al. Time between anterior cruciate ligament injury and reconstruction and cartilage metabolism six-months following reconstruction. Knee 2018; 25 (02) 296-305
    CrossrefPubMedGoogle Scholar
  • 26 Larsson S, Struglics A, Lohmander LS, Frobell R. Surgical reconstruction of ruptured anterior cruciate ligament prolongs trauma-induced increase of inflammatory cytokines in synovial fluid: an exploratory analysis in the KANON trial. Osteoarthritis Cartilage 2017; 25 (09) 1443-1451
    CrossrefPubMedGoogle Scholar
  • 27 Kraus VB, Hargrove DE, Hunter DJ, Renner JB, Jordan JM. Establishment of reference intervals for osteoarthritis-related soluble biomarkers: the FNIH/OARSI OA Biomarkers Consortium. Ann Rheum Dis 2017; 76 (01) 179-185
    CrossrefPubMedGoogle Scholar
  • 28 Garnero P, Piperno M, Gineyts E, Christgau S, Delmas PD, Vignon E. Cross sectional evaluation of biochemical markers of bone, cartilage, and synovial tissue metabolism in patients with knee osteoarthritis: relations with disease activity and joint damage. Ann Rheum Dis 2001; 60 (06) 619-626
    CrossrefPubMedGoogle Scholar
  • 29 Saberi Hosnijeh F, Siebuhr AS, Uitterlinden AG. et al. Association between biomarkers of tissue inflammation and progression of osteoarthritis: evidence from the Rotterdam study cohort. Arthritis Res Ther 2016; 18: 81
    CrossrefPubMedGoogle Scholar
  • 30 Deshpande BR, Katz JN, Solomon DH. et al. Number of Persons With Symptomatic Knee Osteoarthritis in the US: Impact of Race and Ethnicity, Age, Sex, and Obesity. Arthritis Care Res (Hoboken) 2016; 68 (12) 1743-1750
    Google Scholar