RSS-Feed abonnieren
Bitte kopieren Sie die angezeigte URL und fügen sie dann in Ihren RSS-Reader ein.
https://www.thieme-connect.de/rss/thieme/de/10.1055-s-00042133.xml
PDF herunterladen
CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(03): 386-392
DOI: 10.1055/s-0040-1713704
DOI: 10.1055/s-0040-1713704
Original Article
CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries
Autoren
Funding This research was funded by the Directorate of Research and Community Services of Directorate General of Research and Development Strengthening from Ministry of Research, Technology and Higher Education of the Republic of Indonesia.
Abstract
Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC.
Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE+CD35+ and CFSE+CD89+was measured and analyzed using flow cytometry.
Results The expression of CFSE+CD89+ in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p-value of 0.0001, while the expression of CFSE+CD35+ in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group.
Conclusions The expression ratio of CFSE+CD89+ and CFSE+CD35+constitutes a marker for S-ECC.
Publikationsverlauf
Artikel online veröffentlicht:
09. Juli 2020
© .
Thieme Medical and Scientific Publishers Private Ltd.
A-12, Second Floor, Sector -2, NOIDA -201301, India
-
References
- 1 Feldens CA, Giugliani ER, Duncan BB, Drachler MdeL, Vítolo MR. Long-term effectiveness of a nutritional program in reducing early childhood caries: a randomized trial. Community Dent Oral Epidemiol 2010; 38 (04) 324-332
- 2 Suzuki N, Yoneda M, Naito T, Iwamoto T, Hirofuji T. Relationship between halitosis and psychologic status. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106 (04) 542-547
- 3 Zhang X, Yang S, Liao Z. et al. Prevalence and care index of early childhood caries in mainland China: evidence from epidemiological surveys during 1987-2013. Sci Rep 2016; 6: 18897
- 4 Peltzer K, Mongkolchati A. Severe early childhood caries and social determinants in three-year-old children from Northern Thailand: a birth cohort study. BMC Oral Health 2015; 15 (01) 108
- 5 Masumo R, Bardsen A, Mashoto K, Åstrøm AN. Prevalence and socio-behavioral influence of early childhood caries, ECC, and feeding habits among 6-36 months old children in Uganda and Tanzania. BMC Oral Health 2012; 12 (01) 24
- 6 De Stefano R. Psychological factors in dental patient care: odontophobia. Medicina (Kaunas) 2019; 55 (10) 678
- 7 Sachdev J, Bansal K, Chopra R. Effect of comprehensive dental rehabilitation on growth parameters in pediatric patients with severe early childhood caries. Int J Clin Pediatr Dent 2016; 9 (01) 15-20
- 8 Doğan D, Dülgergil CT, Mutluay AT, Yıldırım I, Hamidi MM, Çolak H. Prevalence of caries among preschool-aged children in a central Anatolian population. J Nat Sci Biol Med 2013; 4 (02) 325-329
- 9 Bramantoro T, Karimah N, Sosiawan A. et al. Miswak users’ behavior model based on the theory of planned behavior in the country with the largest Muslim population. Clin Cosmet Investig Dent 2018; 10: 141-148
- 10 Custodio-Lumsden CL, Wolf RL, Contento IR. et al. Validation of an early childhood caries risk assessment tool in a low-income Hispanic population. J Public Health Dent 2016; 76 (02) 136-142
- 11 Califano JV. Research, Science and Therapy Committee American Academy of Periodontology; Position paper: periodontal diseases of children and adolescents. J Periodontol 2003; 74 (11) 1696-1704
- 12 Luthfi M, Setijanto D, Rahardjo MB. et al. Correlation between human neutrophil peptide 1-3 secretion and azurophilic granule (CD63) expression in early childhood caries. Dent Res J (Isfahan) 2019; 16 (02) 81-86
- 13 Elbim C, Katsikis PD, Estaquier J. Neutrophil apoptosis during viral infections. Open Virol J 2009; 3 (01) 52-59
- 14 Mortaz E, Alipoor SD, Adcock IM, Mumby S, Koenderman L. Update on neutrophil function in severe inflammation. Front Immunol 2018; 9: 2171
- 15 Jaillon S, Galdiero MR, Del D Prete, Cassatella MA, Garlanda C, Mantovani A. Neutrophils in innate and adaptive immunity. Semin Immunopathol 2013; 35 (04) 377-394
- 16 Kobayashi SD, Voyich JM, Burlak C, DeLeo FR. Neutrophils in the innate immune response. Arch Immunol Ther Exp (Warsz) 2005; 53 (06) 505-517
- 17 Albrechtsen M, Yeaman GR, Kerr MA. Characterization of the IgA receptor from human polymorphonuclear leucocytes. Immunology 1988; 64 (02) 201-205
- 18 Nobile CG, Fortunato L, Bianco A, Pileggi C, Pavia M. Pattern and severity of early childhood caries in Southern Italy: a preschool-based cross-sectional study. BMC Public Health 2014; 14 (01) 206
- 19 Mayadas TN, Cullere X, Lowell CA. The multifaceted functions of neutrophils. Annu Rev Pathol 2014; 9: 181-218
- 20 Yunanto A, Endharti AT, Widodo A. Neutrophil, TLR2, and TLR4 expression in newborns at risk of sepsis. Paediatr Indonesia 2013; 53 (03) 132
- 21 Gasparoto TH, Vieira NA, Porto VC, Campanelli AP, Lara VS. Differences between salivary and blood neutrophils from elderly and young denture wearers. J Oral Rehabil 2011; 38 (01) 41-51
- 22 Astuti ESY, Sukrama IDM, Mahendra A. Innate immunity signatures of early childhood caries (Ecc) and severe early childhood caries (S-Ecc). Biomed Pharmacol J 2019; 12 (03) 1129-1134
- 23 Borregaard N. Neutrophils, from marrow to microbes. Immunity 2010; 33 (05) 657-670
- 24 Monteiro RC, Van De Winkel JG. IgA Fc receptors. Annu Rev Immunol 2003; 21 (01) 177-204
- 25 Sarantis H, Grinstein S. Subversion of phagocytosis for pathogen survival. Cell Host Microbe 2012; 12 (04) 419-431
- 26 Potempa M, Potempa J. Protease-dependent mechanisms of complement evasion by bacterial pathogens. Biol Chem 2012; 393 (09) 873-888
- 27 Plitas G, Rudensky AY. Regulatory T cells: differentiation and function. Cancer Immunol Res 2016; 4 (09) 721-725
- 28 Yang P, Li Y, Xie Y, Liu Y. Different faces for different places: heterogeneity of neutrophil phenotype and function. J Immunol Res 2019; 2019: 8016254
- 29 Schuster S, Hurrell B, Tacchini-Cottier F. Crosstalk between neutrophils and dendritic cells: a context-dependent process. J Leukoc Biol 2013; 94 (04) 671-675
- 30 Woof JM, Russell MW. Structure and function relationships in IgA. Mucosal Immunol 2011; 4 (06) 590-597
- 31 Luthfi M, Indrawati R, Arundina I, Dachlan YP. Korelasi Jumlah Streptococcus mutans(S. mutans) dan Level Ekspresi Interlukin 8 (IL-8) pada Severe Early Childhood Caries. Maj Kedokt Gigi Indones 2015; 20 (02) 142
- 32 Al Amoudi N, Al Shukairy H, Hanno A. A comparative study of the secretory IgA immunoglobulins (s.IgA) in mothers and children with SECC versus a caries free group children and their mothers. J Clin Pediatr Dent 2007; 32 (01) 53-56
- 33 Miyoshi-Akiyama T, Takamatsu D, Koyanagi M, Zhao J, Imanishi K, Uchiyama T. Cytocidal effect of Streptococcus pyogenes on mouse neutrophils in vivo and the critical role of streptolysin S. J Infect Dis 2005; 192 (01) 107-116
- 34 Brenot A, King KY, Janowiak B, Griffith O, Caparon MG. Contribution of glutathione peroxidase to the virulence of Streptococcus pyogenes. Infect Immun 2004; 72 (01) 408-413