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CC BY-NC-ND 4.0 · Eur J Dent 2020; 14(03): 386-392
DOI: 10.1055/s-0040-1713704
Original Article

CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

Muhammad Luthfi
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Aqsa Sjuhada Oki
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Retno Indrawati
1   Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Muhaimin Rifai
2   Department of Physiology, Cell Culture and Animal Development, Faculty of Sciences, Brawijaya University, Malang, Indonesia
,
Yoes Prijatna Dachlan
3   Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia
,
Fathilah Abdul Razak
4   Department of Oral and Craniofacial Sciences, Faculty of Dentistry, University of Malaya, Kuala Lumpur, Malaysia
› Author Affiliations Funding This research was funded by the Directorate of Research and Community Services of Directorate General of Research and Development Strengthening from Ministry of Research, Technology and Higher Education of the Republic of Indonesia.
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Abstract

Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC.

Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE+CD35+ and CFSE+CD89+was measured and analyzed using flow cytometry.

Results The expression of CFSE+CD89+ in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p-value of 0.0001, while the expression of CFSE+CD35+ in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group.

Conclusions The expression ratio of CFSE+CD89+ and CFSE+CD35+constitutes a marker for S-ECC.

Keywords

CD89 - CD35 - neutrophils - caries - biomarker


Publication History

Article published online:
09 July 2020

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