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CC BY 4.0 · Glob Med Genet 2021; 08(01): 019-023
DOI: 10.1055/s-0041-1723083
Original Article

Evaluation of the Secretor Status of ABO Blood Group Antigens in Saliva using Absorption Inhibition Method

M.L Avinash Tejasvi
1   Kids Research, Nalgonda, Telangana, India
,
Jaya Laksmi Bukkya
2   Department of Oral Medicine and Radiology, Tirumala Dental College, Nizamababad, Telangana, India
,
Pandu Ranga Rao
3   Department of Transfusion Medicine, Kamineni Institute of Medical Sciences, Narketpally, Telangana, India
,
Harsha Bhayya
4   Department of Oral Medicine and Radiology, HKDET Dental College and Hospital Humnabad, Karnataka, India
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Abstract

Objectives While DNA profiling has become the principal technique for individualization of biological evidences, ABO blood grouping is still a useful test method in the initial stages of crime investigation. Objectives of the study were blood group determination using slide agglutination method, blood group determination from saliva using absorption inhibition method, and comparison of slide agglutination method with that of absorption inhibition method from saliva sample.

Materials and Methods A total of 60 subjects were taken randomly with their age ranging from 20 to 60 years. Sixty subjects were divided in to two groups, study group and control group. 5 to 10 mL of unstimulated saliva was collected from 60 patients and Wieners agglutination test was performed to detect the secretor status of blood using absorption inhibition method and compared with that of slide agglutination method

Results Out of 60 subjects, 52 subjects showed secretors of antigen in saliva with percentage value of 86.66% and eight subjects were nonsecretors (13.33%). Slightly higher percentage of secretor status was seen in males 84.6 and 88.2% in females.

Conclusion Evaluation of secretor status of blood group antigen from saliva using absorption inhibition method can be useful method in identification of medicolegal cases.

Keywords

absorption inhibition method - slide agglutination method - secretors


Publication History

Article published online:
23 February 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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