Download PDF
CC BY 4.0 · Glob Med Genet 2021; 08(02): 057-061
DOI: 10.1055/s-0041-1723084
Original Article

Individuals Age Determination from Human Dental Pulp Through DNA Analysis by PCR

M.L. Avinash Tejasvi
1   Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
,
Anulekha Avinash CK
2   Department of Prosthodontics, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
,
E. Rajendra Reddy
3   Department of Pedodontics, Kamineni Institute Dental Sciences, Narketpally, Telangana, India
,
Pavan Kulkarni
4   Department of Oral Pathology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
,
Harsha Bhayya
1   Department of Oral Medicine and Radiology, Kamineni Institute of Dental Sciences, Narketpally, Telangana, India
,
Manohar S. Kugaji
5   Department of Genetics, Central Research Laboratory, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India
› Author Affiliations
› Further Information
   Permissions and Reprints

Abstract

Objectives Age estimation in forensic odontology is having a great importance in recent times because of the request by court or other government authorities so that immigrants whose real age is unknown should not suffer unfair disadvantages because of their supposed age, and so that all legal procedures to which an individual's age is relevant can be properly followed.

Purpose The present study was planned to be conducted on pulp tissue and dental hard tissues derived from individuals for DNA isolation and age determination .

Materials and Methods The present study was an experimental single-blinded study consisting of 30 extracted teeth categorized into three groups as follows: Group A: 10 to 20 years, Group B: 21 to 30 years, Group C: 31 to 40 years. DNA was isolated from the pulp of each tooth and quantitative polymerase chain reaction (qPCR) for calculating telomere length was performed.

Results With increase in age, the length of telomere gets shortened which will be helpful in analyzing the age of the person when morphological and biological remnants are not available except the tooth.

Conclusion The present study found that estimation of human age based on the relative TL measured by the real-time quantitative PCR may be a useful method for age prediction, especially when there is no morphologic information in the biological sample. This is the first study to accesses the age of a person by telomere length using dental pulp.

Keywords

age - PCR - pulp - dentin


Publication History

Article published online:
15 February 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Sun H-W, Gao C-R. The application of telomere DNA in age estimation of forensic medicine. Fa Yi Xue Za Zhi 2005; 21 (02) 155-158
    PubMedGoogle Scholar
  • 2 Vanpelt E, Belkum VAV, Hays JP. Principles and Technical Aspects of PCR Amplification. Netherlands: Springer; 2008: 34
    CrossrefGoogle Scholar
  • 3 Khorate MM, Dhupar A, Ahmed J, Dinkar AD. Gender determination from pulpal tissue. J Forensic Dent Sci 2014; 6 (02) 107-112
    CrossrefPubMedGoogle Scholar
  • 4 O'Callaghan N, Dhillon V, Thomas P, Fenech M. A quantitative real-time PCR method for absolute telomere length. Biotechniques 2008; 44 (06) 807-809
    CrossrefPubMedGoogle Scholar
  • 5 Shay JW, Wright WE. Hallmarks of telomeres in ageing research. J Pathol 2007; 211 (02) 114-123
    CrossrefPubMedGoogle Scholar
  • 6 Ata-Ali J, Ata-Ali F. Forensic dentistry in human identification: a review of the literature. J Clin Exp Dent 2014; 6 (02) e162-e167
    CrossrefPubMedGoogle Scholar
  • 7 Naik PR, Acath DD, Sharma GH, Navalkar AR. Viability of human dental pulp in determination of sex of an individual by identifying SRY gene through DNA analysis: a single blind pilot study. JIAOMR 2012; 24: 133-136
    CrossrefGoogle Scholar
  • 8 Khan R, Tejasvi MLA, Paramkusam G. Comparison of gender determination from dental pulp and dentin after exposure to various environmental conditions: a polymerase chain reaction-based SRY gene study. Contemp Clin Dent 2019; 10 (02) 256-262
    PubMedGoogle Scholar
  • 9 Battepati PM, Shodan M. Gender determination using primary teeth: a polymerase chain reaction (PCR) study. J Dent Oral Hyg 2013; 5: 77-82
    Google Scholar
  • 10 Shammas MA. Telomeres, lifestyle, cancer, and aging. Curr Opin Clin Nutr Metab Care 2011; 14 (01) 28-34
    CrossrefPubMedGoogle Scholar