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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(03): 574-578
DOI: 10.1055/s-0041-1725574
Original Article

Antimicrobial Activity of Roselle-capped​ Silver​ Nanochip on Aggregatibacter actinomycetemcomitans

Sirorat Wacharanad
1   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Puncharee Thatree
2   Secondary Demonstration School, Naresuan University, Phitsanulok, Thailand
,
Punchaya Yiemwattana
2   Secondary Demonstration School, Naresuan University, Phitsanulok, Thailand
,
Penpitcha Paoprajak
3   Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Pimchanok Ngamsangiam
3   Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Menisa Valyanont
3   Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
,
Ichaya Yiemwattana
1   Department of Preventive Dentistry, Faculty of Dentistry, Naresuan University, Phitsanulok, Thailand
› Author Affiliations Funding This project was supported by the Science Classroom in the University Affiliated School Project (SCiUS) under Naresuan University, Naresuan University Secondary Demonstration School, and the Faculty of Dentistry, Naresuan University. The funding of the SCiUS was provided by the Ministry of Science and Technology , Thailand which was highly appreciated.
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Abstract

Objectives This article aimed to study the effects of the​ roselle-capped​ silver​ nanochip​ ​(SNP-Ro​ chip)​ against Aggregatibacter actinomycetemcomitans, and the toxicity of this film on fibroblast cells to develop this SNP-Ro chip into a local chemical for the treatment of periodontitis in the future.

Materials and Methods Using a microwave-assisted synthesis method, silver​ nanoparticles (SNPs) were prepared from a silver nitrate solution and roselle extract as a reducing and capping agent. Then, SNP-Ro chips were fabricated by mixing a solution of SNP-Ro with alginate gel. The antimicrobial effect of the synthesized SNP-Ro chips was performed by the disc diffusion technique and time kill assay. The cytotoxic effect was also determined by the MTS assay.

Statistical Analysis One-way analysis of variance (ANOVA) and Scheffe’s method were used to analyze the data for this experiment.

Results All three ratios of the SNP-Ro chip produced inhibition zones ranging between 18.75 ± 2.08 and 19.03 ± 2.25 mm. In studying the killing time, the three groups of the SNP-Ro chips completely eradicated A. actinomycetemcomitans within 180 minutes. The percentage of the viable SNP-Ro chip-treated human gingival fibroblasts (HGFs) were significantly increased when compared with the alginate chip-treated cells (p < 0.05).

Conclusion This study developed a new method for the deposition of SNPs in alginate gel to make a thin small chip for the sustained release of the SNPs in a periodontal lesion. Therefore, the SNP-Ro chip has the potential to be developed as an adjunctive locally delivered antimicrobial agent in periodontal therapy.

Keywords

Aggregatibacter actinomycetemcomitans - microwave-assisted synthesis - disc diffusion method - time-kill assay - MTS assay - periodontal therapy - antimicrobial


Publication History

Article published online:
26 May 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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