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Development and Validation of Chromatographic Method for the Standardization of Homeopathic Formulation of Syzygium Cumini
Background Syzygium cumini (Lam.), family Myrtaceae, has a long history of use in folk and traditional systems of indigenous medicine. Many homeopathic formulations of Jamun seeds are available in the market for their crucial usage as an anti-diabetic. Despite the popularity of homeopathic products, a lack of standard quality is a significant impediment in their acceptance. The present study aimed to develop and validate a chromatographic method for the standardization of the homeopathic formulation of Syzygium cumini.
Methods The seeds of Syzygium cumini were studied for physicochemical evaluation and preliminary phytochemical screening. Also, the in-house standard and marketed homeopathic formulations of Syzigium cumini were standardized for pH, total fatty content, total phenolic and flavonoid content, with quantitative high-performance liquid chromatography- photodiode array detector (HPLC-PDA) analysis by using ellagic acid as a marker.
Results The physicochemical characteristics of crude material were found to be within pharmacopeial limits. The phytochemical screening showed the presence of various secondary metabolites. The total phenolic and flavonoid content was higher in the in-house standard than in marketed formulations. A validated quantitative HPLC-PDA analysis showed variations of ellagic acid content in different homeopathic formulations.
Conclusion Physicochemical analysis and the HPLC method for quantitative estimation of ellagic acid can be used to standardize a homeopathic formulation of Syzygium cumini.
• Syzygium cumini was studied for physicochemical evaluation and phytochemical screening.
• HPLC standardization was achieved for in-house and marketed homeopathic mother tincture of Syzygium jambolana.
• Ellagic acid was used as a chemical marker.
• HPLC can be used as a substantial method for the standardization of selected homeopathic mother tincture.
Received: 10 August 2020
Accepted: 25 November 2020
21 May 2021 (online)
© 2021. Faculty of Homeopathy. This article is published by Thieme.
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