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CC BY-NC-ND 4.0 · European Dental Research and Biomaterials Journal 2021; 2(01): 12-16
DOI: 10.1055/s-0041-1727090
Original Article

Analysis of Vitamin D Receptor Gene Polymorphism in Chronic Periodontitis among Saudi Population

Fathy M. Elfasakhany
1   Department of Basic and Clinical Oral Sciences, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia
2   Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt
,
Omaima N. Al-Qahtani
3   Dental Intern Program, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia
,
Asmaa M. Badri
3   Dental Intern Program, Faculty of Dentistry, Umm Al Qura University, Makkah, Saudi Arabia
,
Hala A. Abuelela
4   Department of Periodontology and implant Dentistry, Faculty of Dentistry, Ain Shams University, Egypt
› Author Affiliations Funding None.
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Abstract

Objective Genetic and environmental factors have important roles in the development of periodontitis. We aimed to assess the relation of vitamin D receptor (VDR) ApaI and TaqI polymorphisms and the susceptibility of periodontitis in Saudi population in Makkah region.

Materials and Methods In total, 86 unrelated patients with moderate-to-severe periodontitis and 86 controls were enrolled in this study. Evaluation of the periodontal state was performed by using plaque index, bleeding on probing, probing depth, and attachment loss. Extraction of genomic DNA from peripheral blood and genotyping of VDR gene ApaI G/T (rs7975232) and TaqI T/C (rs731236) polymorphisms were performed by utilizing polymerase chain reaction and restriction digestion.

Results There were statistically significant differences between both groups regarding the mean bleeding on probing, mean probing depth, mean plaque index, and the mean attachment level (p < 0.001) indicating that the matching based on the investigated groups was adequate. The examined populations were in Hardy-Weinberg equilibrium. Analysis of the genotype and allele frequencies of both VDR ApaI and TaqI single nucleotide polymorphisms revealed that they were statistically indifferent between the control group and the periodontitis subjects (p> 0.05).

Conclusion These results suggested that VDR ApaI and TaqI polymorphisms might not be related to the susceptibility of periodontal disease in the Saudi subjects in Makkah region.

Keywords

ApaI - TaqI - gene polymorphism - periodontitis - vitamin D receptor - polymerase chain reaction


Publication History

Article published online:
01 October 2021

© 2021. European Dental Research and Biomaterials Journal. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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