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CC BY-NC-ND 4.0 · Eur J Dent 2021; 15(04): 741-745
DOI: 10.1055/s-0041-1729677
Original Article

In vivo Hemostatic Activity of Jatropha mollissima: A Triple-Blinded, Randomized, Controlled Trial in an Animal Model

Maria Vitoria Oliveira Dantas
1   Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Paula Lima Nogueira
1   Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Filipe de Oliveira Lima
1   Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Denis Candeia Pereira Oliveira
1   Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Emanuelly Nara Severiano Gomes
1   Federal University of Campina Grande, Patos, Paraíba, Brazil
,
José Filipe Bacalhau Rodrigues
2   Academic Unit of Materials Engineering, Federal University of Campina Grande, Campina Grande, Paraíba, Brazil
,
Solomon Kweku Sagoe Amoah
2   Academic Unit of Materials Engineering, Federal University of Campina Grande, Campina Grande, Paraíba, Brazil
,
Rosana Araújo Rosendo
3   Academic Unit of Biological Sciences - Dentistry, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Elizandra Silva da Penha
3   Academic Unit of Biological Sciences - Dentistry, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Antonio Flávio Medeiros Dantas
4   Academic Unit of Veterinary Medicine, Federal University of Campina Grande, Patos, Paraíba, Brazil
,
Marcus Vinícius Lia Fook
2   Academic Unit of Materials Engineering, Federal University of Campina Grande, Campina Grande, Paraíba, Brazil
,
Rogério Lacerda-Santos 
5   Department of Orthodontics, Faculty of Dentistry, Federal University of Juiz de Fora, Governador Valadares, Minas Gerais, Brazil
,
Luanna Abílio Diniz Melquiades de Medeiros
3   Academic Unit of Biological Sciences - Dentistry, Federal University of Campina Grande, Patos, Paraíba, Brazil
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Abstract

Objective The objective of this study was to evaluate the hemostatic activity of the sap from Jatropha mollissima (Pohl) Baill. in rats.

Materials and Methods Twenty-four Wistar rats were randomized into four groups (n = 6): the JM25 and JM40 groups were treated with ethanolic extract from the sap of J. mollissima, in a concentration of 25 and 40 mg·mL1, respectively; the MO group was treated with Monsel’s solution and the control group SC with a 0.9% sodium chloride solution.

Statistical Analysis Data were submitted to the Kurskal–Wallis’ test, followed by Dunn’s post hoc (p < 0.05).

Results There was a significant reduction in the bleeding time of the group from the JM25 extract (p = 0.001) when compared with MO and SC. There were no statistically significant differences between groups JM25 and JM40 (p > 0.05). The JM25 group did not present rebleeding, a result significantly different from the MO group (p = 0.001). Monsel’s solution showed significant bleeding, six times greater than the control group SC.

Conclusion The J. mollissima extract, in the concentration of 25 mg·mL1, showed the highest hemostatic efficiency and was found to be a promising biomaterial for the elaboration of a hemostatic product.

Keywords

hemostasis - phytotherapy - biomaterial


Publication History

Article published online:
24 August 2021

© 2021. European Journal of Dentistry. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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