J Neurol Surg A Cent Eur Neurosurg 2023; 84(02): 116-122
DOI: 10.1055/s-0041-1731750
Original Article

Matrix Metalloproteinase-9 is Involved in the Fibrotic Process in Denervated Muscles after Sciatic Nerve Trauma and Recovery

Oleksii Goncharuk
1   Department of Neurosurgery, Bogomolets National Medical University, Kyiv, Ukraine
2   Department of Histology and Embryology, Bogomolets National Medical University, Kyiv, Ukraine
Artem Tykhomyrov
3   Department of Neurosurgery, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU), Kyiv, Ukraine
Mykhailo Guzyk
3   Department of Neurosurgery, Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine (NASU), Kyiv, Ukraine
Volodymyr Medvediev
1   Department of Neurosurgery, Bogomolets National Medical University, Kyiv, Ukraine
Vitaliy Tsymbaliuk
1   Department of Neurosurgery, Bogomolets National Medical University, Kyiv, Ukraine
Yuri Chaikovsky
2   Department of Histology and Embryology, Bogomolets National Medical University, Kyiv, Ukraine
› Author Affiliations


Fibrosis of the injured muscles is a problem of recovery from trauma and denervation. The aim of the work was to investigate the interconnection of matrix metalloproteinase-9 (ММР-9) activity in denervated muscles with fibrosis and to estimate its role in nerve restoration by the epineurial suture, fibrin-based glue, and polyethylene glycol hydrogel. The activity of matrix metalloproteinases was estimated by gelatin zymography. Collagen density in muscles was determined histochemically. An increased level of the active MMP-9 is associated with the fibrous changes in the denervated skeletal muscles and after an epineurial suture. The use of fibrin glue and polyethylene glycol hydrogel resulted in a lower level of collagen and ММР-9 activity, which may be a therapeutic target in the treatment of neuromuscular lesions, and has value in fibrosis analysis following microsurgical intervention for peripheral nerve reconstruction.

Publication History

Received: 19 April 2020

Accepted: 29 December 2020

Article published online:
08 September 2021

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