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Vet Comp Orthop Traumatol 2022; 35(02): 090-095
DOI: 10.1055/s-0041-1736189
Original Research

Intra-Articular Administration of a Synthetic Lubricin in Canine Stifles

Kei Hayashi
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Alexandria Bourgeois
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Daniel Lopez 
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Brian G. Caserto
2   VetPath Services, Stone Ridge, New York, United States
,
Erin Berthelsen
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Ursula Krotscheck
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Heidi L. Reesink
1   College of Veterinary Medicine, Cornell University, Ithaca, New York, United States
,
Sun Young Kim 
3   Purdue University College of Veterinary Medicine, West Lafayette, Indiana, United States
,
David Putnam
4   Meinig School of Biomedical Engineering, Cornell University, Ithaca, New York, United States
5   Smith School of Chemical and Biomolecular Engineering, Cornell University, Ithaca, New York, United States
› Author Affiliations
Funding This study was supported by Center for Advanced Technology (CAT), State of New York and the National Institutes of Health under Award Number R01 AR066667 (to DP). This work made use of the Cornell Center for Materials Research Facilities supported by the National Science Foundation under Award Number DMR-1719875.
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Abstract

Objective The aim of this study was to evaluate the functional, systemic, synovial and articular changes after intra-articular administration of a synthetic lubricin within healthy canine stifles.

Study Design A prospective randomized blinded placebo-controlled study composed of 10 dogs equally divided into either a treatment group (intra-articular synthetic lubricin injection, n = 5) or control group (saline, n = 5). Clinical (orthopaedic examination, gait observation, gait analysis), biochemical (complete blood count and biochemistry profile) and local tissue outcomes (joint fluid analysis, joint capsule and articular cartilage histopathology) were evaluated over a time period of 3 months.

Results No significant differences between the treatment group and control group were identified with regard to baseline patient parameters. No clinically significant orthopaedic examination abnormalities, gait abnormalities, biochemical alterations, joint fluid alterations or histopathological alterations were identified over the course of the study.

Conclusion The synthetic lubricin studied herein is both biocompatible and safe for a single administration within the canine stifle joint. Further research is necessary to evaluate the clinical efficacy of the synthetic lubricin in canine osteoarthritic joints.

Keywords

osteoarthritis - biocompatibility - dogs - stifle

Authors' Contributions

K.H. designed, directed and performed the project. A.B. performed the experiment and organized the data. D.L. organized the data and wrote the manuscript. B.G.C. conducted histopathological analysis. E.B. performed the experiment and organized the data. U.K. and S.Y.K. designed the experimental model and analysed the data. H.R. conceived the original idea, devised the project and planned the experiments. D.P. conducted sample preparation, worked out the technical details and contributed to the interpretation of the results.


Supplementary Material



Publication History

Received: 10 December 2020

Accepted: 16 August 2021

Article published online:
01 October 2021

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