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CC BY 4.0 · European Dental Research and Biomaterials Journal 2021; 02(02): 047-051
DOI: 10.1055/s-0041-1739398
Original Article

Incorporation of Hydroxyapatite and Calcium Triphosphate in the Epoxy Resin-Based Sealer

Ahmed Adel Al-Quraini 
1   College of Dentistry, King Faisal University, Al Hofuf, Saudi Arabia
,
Abdulrahman Fahad Al-Aodah 
2   Royal Commission Health Services, Al-Jubail, Saudi Arabia
,
Adam Ahmed M. Al-Qadhi 
3   Dental Hospital King Faisal University, Al Hofuf, Saudi Arabia
,
Abdul Majeed Munir Ahmad 
4   College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
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Abstract

Objective This study aims to assess and compare the influence of hydroxyapatite (HA) and tricalcium phosphate (TCP) nanoparticles on a commercially available epoxy resinbased sealer, focusing on porosity and push-out bond strength.

Materials and Methods This work was classified into a control group and two experimental groups. In each experimental group, the sealer was mixed with 2.5 wt.% of HA and TCP nanoparticles. Thirty extracted single-rooted teeth were utilized. After sectioning the crowns, the remaining roots of 15 teeth were used, up to 40 to 0.06, using a K3 rotary system. Smear layers were removed with 3 mL of 17% EDTA applied for 60 seconds. Then, the canals were irrigated with 3 mL of 2.25% NaOCl and 5 mL of distilled water. The strength of push-out bonds was tested via an Instron universal testing machine on a 2 mm section acquired from obturated canals. Data were assessed using one-way ANOVA and post hoc tests at a significance level of p < 0.05.

Results A nonsignificant difference (p > 0.05) was evident when the three groups were crosschecked in terms of void volume and bond strength. Micro-CT evaluations revealed the lowest volume of voids to be 0.1152 mm3 (2.69%) for the HA group compared with the control group 0.1818 mm3 (3.9%) and the TCP group 0.2194 mm3 (4.33%). Mean bond strength values were 4.18 ± 1.77 MPa for group 1 (control), 4.19 ± 1.54 MPa for group 2 (HA 2.5%) and 3.76 ± 1.95 MPa for group 3 (TCP 2.5%). Groups 1 and 3 showed both cohesive and a mixed type of failure, while group 2 showed adhesive and a mixed type of bond failure.

Conclusion Within the limitations of the study, incorporation of 2.5 wt% HA and TCP nanoparticles into AH Plus did not significantly affect the percentage volume of voids and the bond strength negatively.

Keywords

bond strength - endodontics - hydroxyapatite - nanoparticles - root canal treatment


Publication History

Article published online:
13 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/)

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  • References

  • 1 Shahi S, Rahimi S, Yavari HR. et al. Effects of various mixing techniques on push-out bond strengths of white mineral trioxide aggregate. J Endod 2012; 38 (04) 501-504
    CrossrefPubMedGoogle Scholar
  • 2 Siddiqui FA, Sheikh A, Rashid S. Radiographic evaluation of dentin thickness and pulp space width for different age groups. J Pak Dent Assoc 2012; 1 (02) 99-102
    Google Scholar
  • 3 Moorer WR, Genet JM. Antibacterial activity of gutta-percha cones attributed to the zinc oxide component. Oral Surg Oral Med Oral Pathol 1982; 53 (05) 508-517
    CrossrefPubMedGoogle Scholar
  • 4 Razavian H, Barekatain B, Shadmehr E, Khatami M, Bagheri F, Heidari F. Bacterial leakage in root canals filled with resin-based and mineral trioxide aggregate-based sealers. Dent Res J (Isfahan) 2014; 11 (05) 599-603
    PubMedGoogle Scholar
  • 5 Alenzi A, Samran A, Samran A. et al. Restoration strategies of endodontically treated teeth among dental practitioners in Saudi Arabia. A nationwide pilot survey. Dent J (Basel) 2018; 6 (03) 44
    CrossrefPubMedGoogle Scholar
  • 6 Tyagi S, Mishra P, Tyagi P. Evolution of root canal sealers: an insight story. Eur J Gen Dent 2013; 2 (03) 199-218
    Article in Thieme ConnectGoogle Scholar
  • 7 Lottanti S, Tauböck TT, Zehnder M. Shrinkage of backfill gutta-percha upon cooling. J Endod 2014; 40 (05) 721-724
    CrossrefPubMedGoogle Scholar
  • 8 Masudi S, Luddin N, Mohamad D, Alkashakhshir J, Adnan R, Ramli R. Eds. In vitro Study on Apical Sealing Ability of Nano-Hydroxyapatite-Filled Epoxy Resin Based Endodontic Sealer. Presented in: AIP Conference Proceedings; 2010: American Institute of Physics
    CrossrefGoogle Scholar
  • 9 Khurshid Z, Zafar MS, Hussain S, Fareed A, Yousaf S, Sefat F. Silver-substituted hydroxyapatite. In: Handbook of Ionic Substituted Hydroxyapatites. Elsevier; London, UK: 2020: 237-257
    CrossrefGoogle Scholar
  • 10 Wang L, Xie X, Li C. et al. Novel bioactive root canal sealer to inhibit endodontic multispecies biofilms with remineralizing calcium phosphate ions. J Dent 2017; 60: 25-35
    CrossrefPubMedGoogle Scholar
  • 11 Kececi AD, Ureyen Kaya B, Adanir N. Micro push-out bond strengths of four fiber-reinforced composite post systems and 2 luting materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 105 (01) 121-128
    CrossrefPubMedGoogle Scholar
  • 12 Rahoma A, AlShwaimi E, Majeed A. Push-out bond strength of different types of mineral trioxide aggregate in root dentin. Int J Health Sci (Qassim) 2018; 12 (05) 66-69
    PubMedGoogle Scholar
  • 13 Al-Haddad AY, Kutty MG, Che Ab Aziz ZA. Push-out bond strength of experimental apatite calcium phosphate based coated gutta-percha. Int J Biomater 2018;2018:1731857
  • 14 Lee JK, Kwak SW, Ha J-H, Lee W, Kim H-C. Physicochemical properties of epoxy resin-based and bioceramic-based root canal sealers. Bioinorg Chem Appl. 2017. 2017. 2582849 DOI: 10.1155/2017/2582849
    CrossrefGoogle Scholar
  • 15 Collares FM, Leitune VC, Rostirolla FV, Trommer RM, Bergmann CP, Samuel SM. Nanostructured hydroxyapatite as filler for methacrylate-based root canal sealers. Int Endod J 2012; 45 (01) 63-67
    CrossrefPubMedGoogle Scholar
  • 16 Bae WJ, Chang SW, Lee SI, Kum KY, Bae KS, Kim EC. Human periodontal ligament cell response to a newly developed calcium phosphate-based root canal sealer. J Endod 2010; 36 (10) 1658-1663
    CrossrefPubMedGoogle Scholar
  • 17 Baras BH, Sun J, Melo MAS. et al. Novel root canal sealer with dimethylaminohexadecyl methacrylate, nano-silver and nano-calcium phosphate to kill bacteria inside root dentin and increase dentin hardness. Dent Mater 2019; 35 (10) 1479-1489
    CrossrefPubMedGoogle Scholar
  • 18 Zafar MS, Alnazzawi AA, Alrahabi M, Fareed MA, Najeeb S, Khurshid Z. Nanotechnology and nanomaterials in dentistry. In: Advanced Dental Biomaterials. Elsevier; London, UK: 2019: 477-505
    Google Scholar
  • 19 Witjaksono W, Naing L, Mulyawati E, Samsudin A, Oo MMT. Sealing ability of hydroxyapatite as a root canal sealer: in vitro study. Dent J (Maj Ked Gigi) 2007; 40 (03) 101-105
    CrossrefGoogle Scholar
  • 20 Toledano M, Muñoz-Soto E, Aguilera FS. et al. The mineralizing effect of zinc oxide-modified hydroxyapatite-based sealer on radicular dentin. Clin Oral Investig 2020; 24 (01) 285-299
    CrossrefPubMedGoogle Scholar
  • 21 Akman M, Akman S, Derinbay O, Belli S. Evaluation of gaps or voids occurring in roots filled with three different sealers. Eur J Dent 2010; 4 (02) 101-109
    Article in Thieme ConnectPubMedGoogle Scholar
  • 22 Toledano M, Muñoz-Soto E, Aguilera FS. et al. A zinc oxide-modified hydroxyapatite-based cement favored sealing ability in endodontically treated teeth. J Dent 2019; 88: 103162
    CrossrefPubMedGoogle Scholar
  • 23 Ali S, Sangi L, Kumar N, Kumar B, Khurshid Z, Zafar MS. Evaluating antibacterial and surface mechanical properties of chitosan modified dental resin composites. Technol Health Care 2020; 28 (02) 165-173
    CrossrefPubMedGoogle Scholar
  • 24 Mohammadian F, Farahanimastary F, Dibaji F, Kharazifard MJ. Scanning electron microscopic evaluation of the sealer-dentine interface of three sealers. Iran Endod J 2017; 12 (01) 38-42
    PubMedGoogle Scholar
  • 25 Ungor M, Onay EO, Orucoglu H. Push-out bond strengths: the Epiphany-Resilon endodontic obturation system compared with different pairings of Epiphany, Resilon, AH Plus and gutta-percha. Int Endod J 2006; 39 (08) 643-647
    CrossrefPubMedGoogle Scholar