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CC BY-NC-ND 4.0 · International Journal of Epilepsy 2021; 07(02): 052-058
DOI: 10.1055/s-0041-1742275
Original Article

Anchusa italica Retz. Hydro-Alcoholic Extract through Attenuation of Oxidative Stress Exerts an Anticonvulsant Effect on the Pentylenetetrazole-Induced Seizure in Mice

Mohammad Rahimi-Madiseh   *
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Shakiba Nasiri-Boroujeni   *
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Zahra Lorigooini
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Meysam Nazari
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Reihaneh Sadeghian
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Mahmoud Rafieian-Kopaei
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
,
Hossein Amini-Khoei 
1   Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
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Abstract

Introduction It has been shown that oxidative stress is involved in the pathophysiology of seizure. Current anticonvulsants have side effects, thus further studies are needed to find new agents with low side effects. Anchusa italica Retz. has been suggested to have antioxidant and neuroprotective effects. The present study aimed to determine the anticonvulsant effects of A. italica hydro-alcoholic extract on pentylenetetrazole (PTZ)-induced seizures in mice focusing on its possible antioxidative stress properties.

Materials and Methods Sixty mice were randomly divided into six groups. The intervention groups received the A. italica extract at the doses of 50, 100, and 200 mg/kg, 30 minutes before the injection of PTZ, whereas the positive control group received phenobarbital. The seizure threshold was then recorded.

Results A. italica extract significantly increased the seizure threshold. The extract significantly increased serum and prefrontal cortex total antioxidant capacity compared with the control group. The A. italica extract significantly reduced serum and prefrontal cortex malondialdehyde and nitrite levels compared with the control group.

Conclusions The results showed the anticonvulsant effects of A. italica extracts on the PTZ-induced seizure in mice is partially due to the attenuation of oxidative stress.

Keywords

seizure - Anchusa italica Retz. extract - oxidative stress - mice

* These are the co-first authors.




Publication History

Article published online:
18 February 2022

© 2022. Indian Epilepsy Society. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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