CC BY-NC-ND 4.0 · Journal of Health and Allied Sciences NU 2022; 12(04): 427-440
DOI: 10.1055/s-0042-1743191
Original Article

Evaluation of the Cariogenic Potential and Total Antioxidant Capacity of Saliva after the Consumption of Candies and Paneer: An In Vivo Study

1   Pediatric Dentist, Mumbai, Maharashtra, India
,
Manju R.
2   Department of Pediatric and Preventive Dentistry, Attavar Balakrishna Shetty Memorial Institute of Dental Sciences, Nitte (Deemed to University), Derelakatte, Mangalore, Karnataka, India
,
Aum B. Joshi
3   College of Dental Sciences, Amargadh, Gujarat, India
,
4   Department of Pediatric and Preventive Dentistry, Attavar Balakrishna Shetty Memorial Institute of Dental Sciences, Derelakatte, Mangalore, Karnataka, India
› Author Affiliations

Abstract

Aim The present study was performed to assess and compare the changes in pH, calcium, phosphorus, alkaline phosphatase, and the total antioxidant capacity (TAC) of saliva after consuming candies, followed by rinsing with water and consumption of paneer.

Materials and Methods Baseline saliva of 5 mL was collected from 60 children and they were asked to chew on candies. After 10 minutes of intake of candies, 5 mL of saliva was collected. They were then divided into two groups of 30 each and test products were given. Group 1: rinse with water and group 2: chew 20 g of paneer and swallow. Saliva of % mL was collected after 10, 20, and, 30 minutes from the study groups. The saliva samples collected were assessed for pH, calcium, phosphorus, alkaline phosphatase, and TAC. The data were analyzed using the paired t-test and post hoc Tukey's test.

Results The pH, calcium, phosphorus, and alkaline phosphatase decreased from baseline values after consuming candies significantly (p < 0.001). These increased after rinsing with water and consumption of paneer which was statistically significant (p < 0.001). The TAC values increased significantly after consuming candies and decreased after rinsing with water and consumption of paneer which was substantial for both the groups.

Conclusion Consumption of candies created a cariogenic milieu. Both the study groups were effective in the reversal of the cariogenic potential. The consumption of paneer caused remineralizing properties and showed superior results than rinsing with water.



Publication History

Article published online:
25 February 2022

© 2022. Nitte (Deemed to be University). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 Dental caries in the child and adolescent. In: Dean JA, Jones JE, Vinson LAW. eds. McDonald and Avery's Dentistry for the child and adolescent. 10th ed.. St. Louis, MO: Elsevier; 2015: 155-176
  • 2 Luke GA, Gough H, Beeley JA, Geddes DA. Human salivary sugar clearance after sugar rinses and intake of foodstuffs. Caries Res 1999; 33 (02) 123-129
  • 3 Janakiram C, Antony B, Joseph J, Ramanarayanan V. Prevalence of dental caries in India among the WHO index age groups: a meta-analysis. J Clin Diagn Res 2018; 12 (08) ZE08-ZE13
  • 4 Sarumathi T, Kumar S, Datta M, Hemalatha VT. Prevalence, severity and associated factors of dental caries in 3-6 year old children. J Clin Diagn Res 2013; 7 (08) 1789-1792
  • 5 Hegde AM, Shetty R, Sequeira AR. The acidogenicity of various chocolates available in the Indian market: a comparative study. Int J Clin Pediatr Dent 2009; 2 (02) 20-24
  • 6 Nirmala S, Quadar MA, Veluru S. pH modulation and salivary sugar clearance of different chocolates in children: a randomized clinical trial. J Indian Soc Pedod Prev Dent 2016; 34 (01) 10-16
  • 7 Morrissey RB, Burkholder BD, Tarka Jr SM. The cariogenic potential of several snack foods. J Am Dent Assoc 1984; 109 (04) 589-591
  • 8 Aruna S, Meenakshi B, Rama KV, Valarmathi S. Salivary levels of calcium and phosphorus in children with and without early childhood caries: A pilot study. SRM J Res Dent Sci 2020; 11: 72-75
  • 9 Pyati SA, Naveen Kumar R, Kumar V, Praveen Kumar NH, Parveen Reddy KM. Salivary flow rate, pH, buffering capacity, total protein, oxidative stress and antioxidant capacity in children with and without dental caries. J Clin Pediatr Dent 2018; 42 (06) 445-449
  • 10 Sruthi KS, Yashoda R, Manjunath P. Diagnostic potential of saliva as a biomarker in early childhood caries: a review. Int J Appl Dent Sci 2019; 5 (02) 341-347
  • 11 Featherstone JD. The science and practice of caries prevention. J Am Dent Assoc 2000; 131 (07) 887-899
  • 12 Kim EE, Wyckoff HW. Reaction mechanism of alkaline phosphatase based on crystal structures. Two-metal ion catalysis. J Mol Biol 1991; 218 (02) 449-464
  • 13 Jain S, Bansal K, Marwaha M, Sehrawat N, Singla S. Effect of diet modification on salivary parameters and oratest in high-caries-risk individuals. Int J Clin Pediatr Dent 2018; 11 (01) 34-39
  • 14 Woodward M, Rugg-Gunn AJ. Chapter 8: milk, yoghurts and dental caries. Monogr Oral Sci 2020; 28: 77-90
  • 15 Johansson I. Milk and dairy products: possible effects on dental health. Scand J Nutr 2002; 46 (03) 119-122
  • 16 Sharma A, Sharma D, Singh S, Sharma A, Sharma R, Sharma M. Milk and its products: effect on salivary pH. Int Healthcare Res J 2018; 2 (06) 140-145
  • 17 Mitoma M, Oho T, Shimazaki Y, Koga T. Inhibitory effect of bovine milk lactoferrin on the interaction between a streptococcal surface protein antigen and human salivary agglutinin. J Biol Chem 2001; 276 (21) 18060-18065
  • 18 Moynihan PJ, Ferrier S, Jenkins GN. The cariostatic potential of cheese: cooked cheese-containing meals increase plaque calcium concentration. Br Dent J 1999; 187 (12) 664-667
  • 19 Sönmez IS, Aras S. Effect of white cheese and sugarless yoghurt on dental plaque acidogenicity. Caries Res 2007; 41 (03) 208-211
  • 20 Bowen WH, Pearson SK. Effect of milk on cariogenesis. Caries Res 1993; 27 (06) 461-466
  • 21 Jensen ME, Wefel JS. Effects of processed cheese on human plaque pH and demineralization and remineralization. Am J Dent 1990; 3 (05) 217-223
  • 22 Tayab T, Rai K, Kumari V, Thomas E. Effect of chewing paneer and cheese on salivary acidogenicity: a comparative study. Int J Clin Pediatr Dent 2012; 5 (01) 20-24
  • 23 Schüpbach P, Neeser JR, Golliard M, Rouvet M, Guggenheim B. Incorporation of caseinoglycomacropeptide and caseinophosphopeptide into the salivary pellicle inhibits adherence of mutans streptococci. J Dent Res 1996; 75 (10) 1779-1788
  • 24 Philip N, Walsh L. The potential ecological effects of casein phosphopeptide-amorphous calcium phosphate in dental caries prevention. Aust Dent J 2019; 64 (01) 66-71
  • 25 Hegde AM, Naik N, Kumari S. Comparison of salivary calcium, phosphate and alkaline phosphatase levels in children with early childhood caries after administration of milk, cheese and GC tooth mousse: an in vivo study. J Clin Pediatr Dent 2014; 38 (04) 318-325
  • 26 Weinsier RL, Krumdieck CL. Dairy foods and bone health: examination of the evidence. Am J Clin Nutr 2000; 72 (03) 681-689
  • 27 Sreebny LM. Saliva in health and disease: an appraisal and update. Int Dent J 2000; 50 (03) 140-161
  • 28 de Sousa ET, Lima-Holanda AT, Nobre-Dos-Santos M. Changes in the salivary electrolytic dynamic after sucrose exposure in children with early childhood caries. Sci Rep 2020; 10 (01) 4146
  • 29 Higham SM, Edgar WM. Effects of Parafilm and cheese chewing on human dental plaque pH and metabolism. Caries Res 1989; 23 (01) 42-48
  • 30 Somaraj V, Shenoy RP, Panchmal GS, Jodalli PS, Sonde L, Nagaraj K. Effect of paneer and cheese consumption on salivary acidogenicity and calcium concentration: a comparative study. Oral Health Prev Dent 2018; 16 (02) 169-174
  • 31 Sungkar S, Chismirina S, Nasution AI, Imaduddin HK. The effect of cheese and milk on buffering capacity of saliva in children 10–12 years. JBBBE 2020; 48: 105-110
  • 32 Ravishankar TL, Yadav V, Tangade PS, Tirth A, Chaitra TR. Effect of consuming different dairy products on calcium, phosphorus and pH levels of human dental plaque: a comparative study. Eur Arch Paediatr Dent 2012; 13 (03) 144-148
  • 33 Pandey P, Reddy NV, Rao VA, Saxena A, Chaudhary CP. Estimation of salivary flow rate, pH, buffer capacity, calcium, total protein content and total antioxidant capacity in relation to dental caries severity, age and gender. Contemp Clin Dent 2015; 6 (Suppl. 01) S65-S71
  • 34 Kashket S, DePaola DP. Cheese consumption and the development and progression of dental caries. Nutr Rev 2002; 60 (04) 97-103
  • 35 Pandey RK, Tripathi A, Chandra S, Pandey A. Relation of salivary phosphorus and alkaline phosphatase to the incidence of dental caries in children. J Pedod 1990; 14 (03) 144-146
  • 36 Gandhy M, Damle SG. Relation of salivary inorganic phosphorus and alkaline phosphatase to the dental caries status in children. J Indian Soc Pedod Prev Dent 2003; 21 (04) 135-138
  • 37 Vahedi M, Davoodi P, Goodarzi M. et al. Comparison of salivary ion activity product for hydroxyapatite (IPHA), alkaline phosphatase and buffering capacity of adults according to age and caries severity. J Dent 2012; 13 (04) 139-145
  • 38 Mohammed IJ, Sarhatb ER, Hamied MA, Sarhat TR. Assessment of salivary interleukin (IL)-6, IL-10, oxidative stress, antioxidant status, pH, and flow rate in dental caries experience patients in Tikrit Province. Sys Rev Pharm 2021; 12 (01) 55-59