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CC BY-NC-ND 4.0 · International Journal of Recent Surgical and Medical Sciences 2023; 09(01): 028-033
DOI: 10.1055/s-0042-1743449
Original Article

Histopathological Changes on Liver Architecture Due to Toxic Effect of Chromium with Protective Role of Vitamin E on Albino Rats: A Prospective Study

Hina Kausar
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
,
Alok Tripathi
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
,
Ram Kumar Kaushik
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
,
Satyam Khare
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
,
Shilpi Jain
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
,
Shobhit Raizaday
1   Department of Anatomy, Subharti Medical College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India
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Abstract

Introduction This histopathological study was done with an aim to learn about the biohazardous effects of chromium toxicity with protective role of alpha-tocopherol on liver parenchyma of albino rat when fed orally.

Materials and Methods The study was conducted in the Department of Anatomy at Subharti Medical College, Meerut (Uttar Pradesh), India, in collaboration with Department of Pathology and Pharmacology.

The experiment was conducted as per the guidelines of the Committee for Purpose of Control and Supervision of Experiments on Animals, with registration number 1204/PO/Re/S/2008/CPSEA, and with permission of the Animal Ethical Committee, on 36 adult male rats of ∼60 days of age; 140 ± 20 g of body weight was used; all the experimental animals were kept in polypropylene cage, and fed with pellet diet and water.

Result When exposed to chromium for long, it resulted in toxicity and damaged the cellular components during reduction process of chromium by releasing of free radicals, leading to deoxyribonucleic acid damage, but the chain-breaking antioxidant effects of alpha-tocopherol played an important role by reversing the damage caused by chromium toxicity, hence proved hepatoprotective.

Conclusion Vitamin E (alpha-tocopherol) is proved to be hepatoprotective against hepatotoxic effect of chromium metal.

Keywords

chromium - vitamin E - Kupffer cells - periportal necrosis - Z-proportion test


Publication History

Article published online:
05 July 2022

© 2022. Medical and Surgical Update Society. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

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  • References

  • 1 Susa N, Ueno S, Sugiyama M. Protective effect of vitamin-E on chromium (VI)-induced cytotoxicity and lipid peroxidation in primary culture of rat hepatocytes. Arch Toxicol 1996; 71: 20-24
    CrossrefPubMedGoogle Scholar
  • 2 O'Brien T, Xu J, Patierno SR. Effects of glutathione on chromium-induced DNA crosslinking and DNA polymerase arrest. Mol Cell Biochem 2001; 222 (1-2): 173-182
    CrossrefPubMedGoogle Scholar
  • 3 Wang XF, Xing ML, Shen Y, Zhu X, Xu LH. Oral administration of Cr(VI) induced oxidative stress, DNA damage and apoptotic cell death in mice. Toxicology 2006; 288: 16-23
    CrossrefPubMedGoogle Scholar
  • 4 Brandes EA, Greenaway HT, Stone HEN. Ductility in chromium. Nature 1956; 178: 587 https://doi.org/10.1038/178587a0
    CrossrefGoogle Scholar
  • 5 Bagchi D, Bagchi M, Hassoun EA. Chromium induced excretion of urinary lipid metabolism DNA damage, generation of reactive oxygen species in Sprague-Dawley rats. Comp Biochem Physiol C Toxicol Pharmacol 1995; 110: 177-187
    Google Scholar
  • 6 Patolla A, Barnes C. Oxidative stress, DNA damage induced by CrVI in Dawley rats. Toxicol. 2009; 24: 66-73
    Google Scholar
  • 7 Gale TF. The embryotoxic response to maternal chromium trioxide exposure in different strains of hamsters. Environ Res 1982; 29 (01) 196-203
    CrossrefPubMedGoogle Scholar
  • 8 Anjum F, Shakoori AR. Sublethal effects of hexavalent chromium on the body growth rate and liver function enzymes of phenobarbitone-pretreated and promethazine-pretreated rabbits. J Environ Pathol Toxicol Oncol 1997; 16 (01) 51-59
    PubMedGoogle Scholar
  • 9 Costa M. Toxicity & carcinogenicity of hexavalent chromium in animal models & human. Crit Rev Toxicol 1997; 27: 431-442
    CrossrefPubMedGoogle Scholar
  • 10 Acharya S, Mehta K, Krishnan S, Rao CV. A subtoxic interactive toxicity study of ethanol and chromium in male Wistar rats. Alcohol 2001; 23 (02) 99-108
    CrossrefPubMedGoogle Scholar
  • 11 Da Silva RF, Sala MA, De Souza AM. et al. Action of Cr III on rat liver structure, histometric & haematological studies. Int J Morphol 2006; 24: 197-203
    Google Scholar
  • 12 Soudani N, Ben Amara I, Sefi M, Boudawara T, Zeghal N. Effects of selenium on chromium (VI)-induced hepatotoxicity in adult rats. Exp Toxicol Pathol 2011; 63 (06) 541-548
    CrossrefPubMedGoogle Scholar
  • 13 Dwivedi DK, Jena GB. Glibenclamide protects against thioacetamide-induced hepatic damage in Wistar rat: investigation on NLRP3, MMP-2, and stellate cell activation. Naunyn Schmiedebergs Arch Pharmacol 2018; 391 (11) 1257-1274
    CrossrefPubMedGoogle Scholar