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DOI: 10.1055/s-0042-1744242
Unraveling the Possibilities of Monoclonal Protein Migration, Identification, and Characterization in SPEP on Capillary Zone Electrophoresis
Autoren
Abstract
Serum protein electrophoresis (SPEP) is a method by which proteins present in serum are separated into different fractions based on their molecular weight and electric charge. Presence of M spike, composed of monoclonal protein, on electrophoretogram is a characteristic finding that can be seen in monoclonal gammopathies like multiple myeloma. M spike is most commonly seen in the gamma region however, the M-spike can be observed in fraction other than the Y fraction as well i.e. in the beta region and rarely alpha region. Here we have enumerated few cases where M protein has been seen in fractions other than the gamma region. Thus one needs to be cautious about the variable appearance of M-spike during interpretation of SPEP as some physiological proteins if elevated can also give rise to similar spike sometimes referred as pseudo monoclonal pattern.
Keywords
capillary zone electrophoresis - multiple myeloma - M-spike - monoclonal protein - variable migrationAuthors' Contribution
All the authors contributed to the concepts, design, definition of intellectual content, and statistical analysis. S.P., R.S., and R.V. contributed to the literature research, manuscript preparation, and data analysis. S.P., V.K., and R.S. contributed to data acquisition. S.P., V.K., R.S., and R.V. contributed to manuscript editing. R.S., L.C., and B.C.K. contributed to manuscript review. All authors are the guarantors of this paper.
Publikationsverlauf
Artikel online veröffentlicht:
25. April 2022
© 2022. The Indian Association of Laboratory Physicians. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)
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References
- 1 Dash NR, Mohanty B. Multiple myeloma: a case of atypical presentation on protein electrophoresis. Indian J Clin Biochem 2012; 27 (01) 100-102
- 2 Keren DF, Schroeder L. Challenges of measuring monoclonal proteins in serum. Clin Chem Lab Med 2016; 54 (06) 947-961
- 3 Tripathy S. The role of serum protein electrophoresis in the detection of multiple myeloma: an experience of a corporate hospital. J Clin Diagn Res 2012; 6 (09) 1458-1461
- 4 Smith JD, Raines G, Schneider HG. Should routine laboratories stop doing screening serum protein electrophoresis and replace it with screening immune-fixation electrophoresis? No quick fixes: counterpoint. Clin Chem Lab Med 2016; 54 (06) 967-971 (CCLM)
- 5 Regeniter A, Siede WH. Peaks and tails: evaluation of irregularities in capillary serum protein electrophoresis. Clin Biochem 2018; 51: 48-55
- 6 Gastélum-Cano JM, Fragoso-Flores J, Noffal-Nuño VM, Deffis-Court M. An unusual pattern in serum protein electrophoresis to take in mind: a case report. Pract Lab Med 2021; 24: e00200
- 7 Plasma proteins. In: Marshall WJ. ed. Illustrated Textbook of Clinical Chemistry. 2nd ed.. London: Gower Medical Publications; 1992: 210-221