RSS-Feed abonnieren
DOI: 10.1055/s-0042-1747070
3D Bio-Printed HNSCC (Head and neck squamous cell carcinoma) in vitro model for biomedical applications
Introduction
The tumor microenvironment (TME) greatly influences the proliferation and progression of HNSCC. Hence, an in depth understanding of the in vivo TME is crucial and recapitulating in vivo like pathophysiology in vitro, still remains a challenge. 3D bioprinted models are increasingly researched on due to their ability to precisely replicate the tumor cellular organization as seen in vivo. This project attempts to replicate the HNSCC TME in vitro via 3D bio printing technique.
Methods
Nanocellulose derived from Tunicates is used as the hydrogel/bio-ink for our 3D bio-printed model due to its commendable biocompatibility and low bioburden/ toxicity to the cells. Initially, the HNSCC cell laden bio-ink is printed in the form of cylindrical constructs to study the cell viability and the stability of the bio-ink in long term cultures. To optimize the bio-printing parameters, the constructs are also printed with different cellular densities and bio-ink concentrations. The distribution of cells in the hydrogel matrix were observed via H&E staining to determine its proliferation and distributions in the hydrogel matrix.
Results
After monitoring constructs for 21 days, a high rate of viable cells (70 – 80%) were observed in constructs printed with high cellular density (10<sup>7 </sup>cells/mL), while low rates of viable cells were observed in constructs printed with low cellular density (10<sup>5</sup>-10<sup>6 </sup>cells/mL).
Conclusion
At a high cell density, cells show a higher and sufficient viability in the bioprinted constructs. As a further step, the stellate cells are to be printed with the tumor cells to study the cell-cell and cell-matrix interaction and their respective biochemical cues. 3D bio-printed HNSCC model could serve as a promising tool in biomedical applications such as drug testing.
Project funding by the state of Baden-Württemberg (grant number 33-7533-6-1522 / 10/4)
Publikationsverlauf
Artikel online veröffentlicht:
24. Mai 2022
© 2022. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).
Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart,Germany