A New Colorimetric DPPH Radical Scavenging Activity Method: Comparison with Spectrophotometric Assay in Some Medicinal Plants Used in Moroccan Pharmacopoeia

Fatiha EL Babili; Clotilde Nigon; Li Lacaze; Juliette Millé; Anthony Masiala; Jennifer Simm; Virginie M. Lamade; Amal Ait El Haj

doi:10.1055/s-0042-1748031

Pharmaceutical Fronts, Inhaltsverzeichnis

CC BY 4.0 · Pharmaceutical Fronts 2022; 04(02): e89-e102
DOI: 10.1055/s-0042-1748031

Original Article

A New Colorimetric DPPH Radical Scavenging Activity Method: Comparison with Spectrophotometric Assay in Some Medicinal Plants Used in Moroccan Pharmacopoeia

Fatiha EL Babili

¹Henri Gaussen Botanical Garden, Paul Sabatier University Toulouse III, Toulouse, France

,

Clotilde Nigon

²ENSAT, National Agronomic School of Toulouse, Castanet-Tolosan, France

,

Li Lacaze

³Master in Engineering Sciences, Institut National Polytechnique of Nancy, Nancy Cedex, France

,

Juliette Millé

⁴Faculty of Engineering Sciences, Paul Sabatier University, Toulouse Cedex, France

,

Anthony Masiala

⁴Faculty of Engineering Sciences, Paul Sabatier University, Toulouse Cedex, France

,

Jennifer Simm

⁴Faculty of Engineering Sciences, Paul Sabatier University, Toulouse Cedex, France

,

Virginie M. Lamade

⁵Jean Jaures Toulouse University, (Toulouse) and Botanista – (Plaisance du Touch), France

,

Amal Ait El Haj

⁶Laboratory of Pharmacognosy, Faculty of Medicine and Pharmacy, Hassan II University, Casablanca, Morocco

› Institutsangaben

Abstract

Antioxidants in medicinal plants are particularly important in protecting against reactive oxygen species (ROS)-related diseases, such as heart and blood vessel disease, nervous system degeneration, and cancer. Because our bodies are not strong enough to completely neutralize ROS, we sometimes need antioxidant supplementation from herbs. There is ample empirical evidence in traditional pharmacopoeias. The antioxidant activities of plant drugs have long been spectrophotometrically measured with 2,2-diphenyl-1-picrylhydrazyl (DPPH). In this study, a new colorimetry DPPH radical scavenging activity method (validated to ICH standards) for some medicinal plants, used in Moroccan pharmacopoeia, was reported and made a comparison with spectrophotometric assay. In the method, a solution of DPPH^• is incubated in the presence of an antioxidant control (Trolox) or medicinal plant extracts in wells on a 96-well plate. After an appropriate reaction time, in the dark, the plate was scanned and images obtained were processed and analyzed by Image J software. This analysis will allow us to evaluate substance's antioxidant activity, almost in the same way as a spectrophotometric assay. The colorimetric DPPH^• method exhibited a strong correlation (R ² > 0.95) with the conventional spectrophotometric DPPH^• method. The colorimetric DPPH^• method had excellent accuracy (103.81–105.47%), precision (1.051–10.85% RSD [relative standard deviation]), reproducibility (1.457% RSD), and robustness (1.05–1.38 F test). The developed DPPH^• test was easy, fast, low cost and reliable, and can be used for high-throughput assay for screening DPP-scavenging activity in herb medicines.

Keywords

colorimetric DPPH^• method - herbal drugs - antioxidant activity - screening - pharmacopoeia

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Referenzen

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