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CC BY 4.0 · Arq Neuropsiquiatr 2022; 80(09): 900-907
DOI: 10.1055/s-0042-1755321
Original Article

Bioflavonoid exerts analgesic and anti-inflammatory effects via transient receptor potential 1 channel in a rat model

Bioflavonoide exerce efeitos analgésicos e anti-inflamatórios via canal receptor do potencial transitório 1 em um modelo de rato
Mohammad Mahdi Zarei 
1   Islamic Azad University, Department of Pharmacology, Karaj, Iran.
,
Zohreh Abdolmaleki 
1   Islamic Azad University, Department of Pharmacology, Karaj, Iran.
,
Siamak Shahidi 
2   University of Medical Sciences, School of Medicine, Department of Physiology, Hamadan, Iran.
3   Hamadan University of Medical Sciences, Neurophysiology Research Center, Hamadan, Iran.
› Author Affiliations Support This research was supported by a grant (No. 1397–115) from the Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Iran.
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Abstract

Background Pain is an uncomfortable sensation in the body. Kaempferol is a flavonoid with antinociceptive effects. Transient receptor potential (TRP) channels have been characterized in the sensory system.

Objective This study evaluated the central antinociceptive effect of Kaempferol and possible mechanisms of action of transient receptor potential cation channel subfamily V member 1 (TRPV1).

Methods Capsaicin as a TRPV agonist (5 μg/μL, intracerebroventricular [ICV]) and capsazepine as its antagonist (10 μg/μL, icv) were used to test the analgesic effect of kaempferol (1.5 mg, ICV). Morphine (10 μg, ICV) was used as a positive control. The other groups were treated with a combination of kaempferol and capsaicin, kaempferol and capsazepine, and capsaicin and capsazepine. The cannula was implanted in the cerebroventricular area. The tail-flick, acetic acid, and formalin tests were used to assess analgesic activity. For evaluation of antiinflammatory effect, the formalin-induced rat paw edema was used.

Results Kaempferol significantly decreased pain in the acute pain models, including the tail-flick and the first phase of the formalin test. In the late phase of the formalin test, as a valid model of nociception, capsazepine inhibited the antinociceptive effect of kaempferol.

Conclusions Kaempferol has an analgesic effect in the acute pain model and can affect inflammatory pain. Also, the TRPV1 channel plays a role in the antinociceptive activity of kaempferol.

Resumo

Antecedentes A dor é uma sensação desconfortável no corpo. Kaempferol é um flavonoide com efeitos antinociceptivos. Canais receptores de potencial transitório têm sido caracterizados no sistema sensorial.

Objetivo Este estudo avaliou o efeito antinociceptivo central do kaempferol e os possíveis mecanismos de ação do TRPV1.

Métodos Capsaicina como agonista de TRPV (5 μg/μL, intracerebroventricular [ICV]) e capsazepina como seu antagonista (10 μg/μL, icv) foram usados para testar o efeito analgésico do kaempferol (1,5 mg, ICV). A morfina (10 μg, ICV) foi usada como controle positivo. Os outros grupos foram tratados com uma combinação de kaempferol e capsaicina, kaempferol e capsazepina e capsaicina e capsazepina. A cânula foi implantada na área cerebroventricular. Os testes de movimento de cauda, ácido acético e formalina foram usados para avaliar a atividade analgésica. Para avaliação do efeito anti-inflamatório, foi utilizado o edema de pata de rato induzido por formalina.

Resultados Kaempferol diminuiu significativamente a dor nos modelos de dor aguda, incluindo o movimento da cauda e a primeira fase do teste de formalina. Na fase tardia do teste da formalina, como modelo válido de nocicepção, a capsazepina inibiu o efeito antinociceptivo do kaempferol.

Conclusões Kaempferol tem efeito analgésico no modelo de dor aguda e pode afetar a dor inflamatória. Além disso, o canal TRPV1 desempenha um papel na atividade antinociceptiva do kaempferol.

Keywords

Analgesia - TRPA1 Cation Channel - Rats - Flavonoids

Palavras-chave

Analgesia - Canal de cátion TRPA1 - Ratos - Flavonoides

Authors' Contributions

All authors: study concept and design, analysis and interpretation of data, and critical revision of the manuscript for important intellectual content; MMZZA: acquisition of data, statistical analysis, and drafting of the manuscript; SS: administrative, technical, and material support; ZA: study supervision.




Publication History

Received: 28 September 2021

Accepted: 26 December 2021

Article published online:
09 November 2022

© 2022. Academia Brasileira de Neurologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/)

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