Abstract
Objective This study determined the potency of resin infiltrations and resin sealant in impeding
microleakage on artificial white-spot lesions (AWL) by methylene blue penetration.
Materials and Methods Eighty AWL specimens were randomly separated into two groups for water storage for
24 hours (groups 1–4) and 20,000 cycles of thermocycling (TC) (groups 5–8). Each group
was then separated into four subgroups (n = 10) based on the AWL surface treatments: (1) no Tx, (2) treated with resin infiltration
(ICON, DMG, Hamburg, Germany), (3) treated with resin infiltration (Surface pre-reacted
glass-ionomer (S-PRG) barrier coat, SHOFU, Kyoto, Japan), (4) treated with resin sealant
(Clinpro sealant, 3M ESPE, Minnesota, United States). Nail varnish was covered to
all samples, with the exception of a 4 × 4 mm2 patch on the buccal measurement region, which was subsequently submerged in a 2%
methylene blue solution and cut into buccolingual sections. Stereomicroscope measurements
were used to calculate methylene blue penetration scores.
Statistical Analysis The Kruskal–Wallis test and the Bonferroni post-hoc correction were performed to
evaluate the data.
Results Application of resin infiltrants and resin sealant reduced microleakage in AWL by
methylene penetration both before and after thermal cycling. In addition, group 6
(ICON + TC) and group 7 (S-PRG + TC) had a significantly different value from group
8 (sealant + TC).
Conclusion Both the resin infiltration approach and the resin sealant seem to help seal AWL
and might perhaps offer long-term defense against microleakage in AWL caused by methylene
blue penetration. The greatest sealing and defense for microleakage in AWL were demonstrated
by the resin infiltrations.
Keywords
methylene blue - microleakage - resin infiltration - resin sealant - white-spot lesion
