Osteologie 2022; 31(04): e6
DOI: 10.1055/s-0042-1757534
Abstracts

Early post-operative exercise promotes bone healing kinetics: In vivo study with rats

Autoren

  • O Suljevic

    1   Department of Orthopedics and Traumatology, Medical University of Graz

  • Y U Schwarze

    1   Department of Orthopedics and Traumatology, Medical University of Graz

  • V Herber

    1   Department of Orthopedics and Traumatology, Medical University of Graz

  • B Okutan

    1   Department of Orthopedics and Traumatology, Medical University of Graz

  • M A Weinberg

    1   Department of Orthopedics and Traumatology, Medical University of Graz

  • G N Sommer

    1   Department of Orthopedics and Traumatology, Medical University of Graz
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Introduction Physical exercise represents a well-known modality for maintaining healthy locomotor mechanism and bones in general [1] [2]. This statement was supported by previous studies which researched the effect of physical exercise on relieving symptoms of osteoporosis, osteoarthritis and other bone-related diseases [3]. Studies which investigated this topic on a preclinical level, demonstrated that treadmill training in rats is a reliable exercise protocol for in depth analysis of bone microstructural changes [4] [5].

Material and Methods Ten Sprague Dawley male rats underwent bi-cortical 1.6 mm hole drilling in both femur diaphysis, after which n=5 underwent continuous treadmill training over two weeks, while other rats (n=5) were entitled to sedentary control group. New bone formation labeling was performed by subcutaneous fluorochrome injections at day 0, 14 and week 5. In vivo micro CT scans were performed after the surgery and then once a week during the 6-week postoperative period after which euthanasia of all animals was performed. Femur samples were extracted and underwent ex vivo scanning and histological evaluation, while serum was used for evaluating bone remodeling markers.

Results Micro-CT data demonstrated increased volume and surface of newly formed bone in defect area of exercise group, as well as enhanced bone healing kinetics. Statistically significant increase was observed after one week in volume of newly formed bone of training in comparison to sedentary group. Alkaline phosphates levels showed increase in both groups, after 6 weeks.

Conclusion Our study demonstrated positive effects of 2-week postoperative treadmill training in terms of enhanced bone healing kinetics and prominent callus formation as confirmed by the radiological methods.



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Artikel online veröffentlicht:
18. Oktober 2022

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